ZFIN ID: ZDB-FIG-140617-20
Flasse et al., 2013 - Ascl1b and Neurod1, instead of Neurog3, control pancreatic endocrine cell fate in zebrafish. BMC Biology   11(1):78 Full text @ BMC Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: Prim-5 to Prim-15

Fig. 7 Impaired endocrine cell differentiation in ascl1b morphants. Ventral views with the anterior part to the left of WISH showing expression of insulin(A-B), somatostatin(C-D), ghrelin(E-F) and glucagon(G-H) in control (A, C, E, G) and ascl1b morphants (B, D, F, H) at 30 hpf. The quantifications on the right side of the figure represent the number of positive cells per embryo for controls and ascl1b morphants. (I-J) Confocal projections of ventral views of fluorescent WISH showing expression of isl1 in control (I) and ascl1b morphants (J) at 24 hpf. The quantification of isl1 expression domain is depicted on the right side of the figure. The graph shows the relative volume of isl1+ cells in control and ascl1b morphants, the mean of the volume occupied by the isl1+ cells in control morphants being arbitrarily set to 100%. Asterisks (***) indicate that the difference between controls and ascl1b morphants is statistically significant by Student’s t-test (P <0.001). Scale bars : 50 µm hpf, hours post fertilization; WISH, whole-mount in situ hybridization.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gcga WT control Prim-15 pancreatic bud ISH
WT + MO1-ascl1b standard conditions Prim-15 pancreatic bud ISH
ghrl WT control Prim-15 pancreatic bud ISH
WT + MO1-ascl1b standard conditions Prim-15 pancreatic bud ISH
ins WT control Prim-15 pancreatic bud ISH
WT + MO1-ascl1b standard conditions Prim-15 pancreatic bud ISH
isl1a WT control Prim-5 pancreatic bud ISH
WT + MO1-ascl1b standard conditions Prim-5 pancreatic bud ISH
sst2 WT control Prim-15 pancreatic bud ISH
WT + MO1-ascl1b standard conditions Prim-15 pancreatic bud ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Biol.