ZFIN ID: ZDB-FIG-140617-21
Flasse et al., 2013 - Ascl1b and Neurod1, instead of Neurog3, control pancreatic endocrine cell fate in zebrafish. BMC Biology   11(1):78 Full text @ BMC Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 5-9 somites to 10-13 somites

Fig. 8 The onset of sox4b expression is delayed in ascl1b morphants and is restricted to neurod1 expressing cells. Ventral views with the anterior part to the left of double fluorescent WISH showing expression of sox4b and neurod1 at 12 hpf (A-F), 13 hpf (G-L) and 17 hpf (M-R) in control and ascl1b morphants. The domain occupied by sox4b+ and neurod1+ cells was reduced respectively 3.3- and 2.6-fold in the Mo-ascl1b at 17 hpf. The arrows point to a cell expressing sox4b and neurod1 while the arrowheads points to a cell expressing only sox4b. Z-plane confocal images. Scale bars : 50 ┬Ám hpf, hours post fertilization; WISH, whole-mount in situ hybridization.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
neurod1 WT control 5-9 somites trunk embryonic structure ISH
10-13 somites pancreas primordium ISH
WT + MO1-ascl1b standard conditions 5-9 somites trunk embryonic structure ISH
10-13 somites pancreas primordium ISH
sox4b WT control 5-9 somites trunk embryonic structure ISH
10-13 somites pancreas primordium ISH
WT + MO1-ascl1b standard conditions 5-9 somites trunk embryonic structure ISH
10-13 somites pancreas primordium ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Biol.