ZFIN ID: ZDB-FIG-140617-23
Flasse et al., 2013 - Ascl1b and Neurod1, instead of Neurog3, control pancreatic endocrine cell fate in zebrafish. BMC Biology   11(1):78 Full text @ BMC Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-25
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-25

Fig. 10 Endocrine cell differentiation is abolished in the double ascl1b and neurod1 morphants. Ventral views of WISH showing expression of insulin(A-B, I-J), somatostatin(C-D), ghrelin(E-F) and glucagon(G-H) in control (A,C,E,G,I) and Mo-ascl1b/Mo2-neurod1(B,D,F,H,J) morphants at 38 hpf (A-H) or 17 hpf (I-J). The quantifications on the right side of the figure represent the number of positive cells per embryo for controls and double ascl1b/neurod1 morphants. Asterisks (***) indicate that the difference between cell number in controls and double ascl1b/neurod1 morphants is statistically significant by Student’s t-test (P <0.001). hpf, hours post fertilization; WISH, whole-mount in situ hybridization.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
gcga WT control Prim-25 pancreatic bud ISH
WT + MO1-ascl1b + MO3-neurod1 standard conditions Prim-25 Not Detected pancreatic bud ISH
ghrl WT control Prim-25 pancreatic bud ISH
WT + MO1-ascl1b + MO3-neurod1 standard conditions Prim-25 Not Detected pancreatic bud ISH
ins WT control 14-19 somites trunk embryonic structure ISH
Prim-25 pancreatic bud ISH
WT + MO1-ascl1b + MO3-neurod1 standard conditions 14-19 somites Not Detected trunk embryonic structure ISH
Prim-25 Not Detected pancreatic bud ISH
sst2 WT control Prim-25 pancreatic bud ISH
WT + MO1-ascl1b + MO3-neurod1 standard conditions Prim-25 Not Detected pancreatic bud ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-ascl1b + MO3-neurod1 standard conditions Prim-25 endocrine pancreas development disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Biol.