ZFIN ID: ZDB-FIG-140617-22
Flasse et al., 2013 - Ascl1b and Neurod1, instead of Neurog3, control pancreatic endocrine cell fate in zebrafish. BMC Biology   11(1):78 Full text @ BMC Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 5-9 somites to Prim-25
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 14-19 somites to Prim-25

Fig. 9 Simultaneous knock-down of Ascl1b and Neurod1 blocks the differentiation process of the precursors. Ventral views with the anterior part to the left of the pancreas of control (A,C,E,G,I) or Mo-ascl1b/Mo2-neurod1 morphants (B,D,F,H,J) analyzed by fluorescent (A-D, G-J) or visible (E-F) WISH. (A-B) Confocal projections of isl1 expression in the pancreatic islet (dotted line) and in the adjacent mesoderm (*) at 38 hpf (C-D) and of sox4b expression at 17 hpf. (E-F)ascl1b transcript expression at 13 hpf (8s). (G-H) Confocal projections of pax6b (green) and mnx1 (red) expression at 17.5 hpf showing that the pancreatic expression of pax6b and mnx1 is completely lost in the double morphants. In contrast, the expression of pax6b in the neural tube and the expression of mnx1 gene in the motoneurons and the hypochord is not perturbed. (I-J)neurod1 transcript expression at 15 hpf (12s). hpf, hours post fertilization; WISH, whole-mount in situ hybridization.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ascl1b WT control 5-9 somites trunk embryonic structure ISH
WT + MO1-ascl1b + MO3-neurod1 standard conditions 5-9 somites trunk embryonic structure ISH
isl1a WT control Prim-25 posterior pancreatic bud ISH
WT + MO1-ascl1b + MO3-neurod1 standard conditions Prim-25 Not Detected posterior pancreatic bud ISH
mnx1 WT control 14-19 somites pancreas primordium ISH
WT + MO1-ascl1b + MO3-neurod1 standard conditions 14-19 somites pancreas primordium ISH
neurod1 WT control 10-13 somites pancreas primordium ISH
WT + MO1-ascl1b + MO3-neurod1 standard conditions 10-13 somites pancreas primordium ISH
pax6b WT control 14-19 somites pancreas primordium ISH
WT + MO1-ascl1b + MO3-neurod1 standard conditions 14-19 somites Not Detected pancreas primordium ISH
sox4b WT control 14-19 somites pancreas primordium ISH
WT + MO1-ascl1b + MO3-neurod1 standard conditions 14-19 somites Not Detected pancreas primordium ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-ascl1b + MO3-neurod1 standard conditions 14-19 somites endocrine pancreas development disrupted, abnormal
Prim-25 endocrine pancreas development disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Biol.