ZFIN ID: ZDB-FIG-140617-14
Flasse et al., 2013 - Ascl1b and Neurod1, instead of Neurog3, control pancreatic endocrine cell fate in zebrafish. BMC Biology   11(1):78 Full text @ BMC Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-15 to Protruding-mouth

Fig. 1 neurog3 is not expressed in the pancreas and null neurog3 mutant embryos do not display any apparent endocrine defects. (A-B) Whole-mount in situ hybridization (WISH) showing expression of neurog3 in the hypothalamus at 30 hpf (A) and in scattered cells of the gut (dotted lines) at 72 hpf (B). (C) WISH showing that the number of cells expressing insulin, somatostatin, ghrelin and glucagon is not changed in the neurog3 sa211 mutants compared to the wt siblings. All views are ventral with the anterior part to the left. Scale bars : 50 ┬Ám hpf, hours post fertilization; wt, wild type.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gcga WT standard conditions Prim-15 pancreatic bud ISH
neurog3sa211/sa211 standard conditions Prim-15 pancreatic bud ISH
ghrl WT standard conditions Long-pec pancreas ISH
neurog3sa211/sa211 standard conditions Long-pec pancreas ISH
ins WT standard conditions Prim-15 pancreatic bud ISH
neurog3sa211/sa211 standard conditions Prim-15 pancreatic bud ISH
neurog3 WT standard conditions Prim-15 hypothalamus ISH
Protruding-mouth gut ISH
sst2 WT standard conditions Prim-15 pancreatic bud ISH
neurog3sa211/sa211 standard conditions Prim-15 pancreatic bud ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Biol.