FIGURE

Fig. 4

ID

ZDB-FIG-080506-4

Publication

Hong et al., 2008 - The Mych Gene Is Required for Neural Crest Survival during Zebrafish Development

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Fig. 4

Mych MO affects multiple regions at the 3-somite stage.
A?P. Lateral views (A?H) and dorsal views (I?P) of control MO-injected (A,C,E,G,I,K,M) and mych MO-injected embryos (B,D,F,H,J,L,N). Mab21l2 (A?B′) as eye anlage and midbrain marker; dmbx1a (C?D′) as eye territory and tectum marker; barhl2 (E,F) as diencephalon marker; rx3 as eye field marker. (I?J) double label with pax2a (red) and egr2b (blue). (K?P). Embryos were examined using four probes, ctsl1b (red), dlx3b (blue), ntl (blue), and pcdh8 (red). (O?P) mych MO-induced defects were rescued by co-injection with mych mRNA. lnp, lateral neural plate; mi, midbrain; n, notochord; pp, prechordal plate; r, rhombomere; psm, pre somitic mesoderm.

Expression Data

Genes:	barhl2 ctslb dlx3b dmbx1a egr2b mab21l2 pax2a pcdh8 rx3 tbxta (all 10)
Fish:	AB AB + MO1-mych
Knockdown Reagent:	MO1-mych
Anatomical Terms:	midbrain neural keel neural plate notochord optic primordium prechordal plate presumptive diencephalon presumptive rhombomere 3 presumptive rhombomere 5 segmental plate (all 9)
Stage:	1-4 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-mych
Knockdown Reagent:	MO1-mych
Observed In:	eye hindbrain midbrain trunk
Stage:	1-4 somites

Phenotype Detail

Acknowledgments

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Open Access

Full text @ PLoS One