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ZFIN ID: ZDB-FIG-080506-4
Hong et al., 2008 - The Mych Gene Is Required for Neural Crest Survival during Zebrafish Development. PLoS One   3(4):e2029 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: 1-4 somites
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: 1-4 somites

Fig. 4 Mych MO affects multiple regions at the 3-somite stage.
A–P. Lateral views (A–H) and dorsal views (I–P) of control MO-injected (A,C,E,G,I,K,M) and mych MO-injected embryos (B,D,F,H,J,L,N). Mab21l2 (A–B′) as eye anlage and midbrain marker; dmbx1a (C–D′) as eye territory and tectum marker; barhl2 (E,F) as diencephalon marker; rx3 as eye field marker. (I–J) double label with pax2a (red) and egr2b (blue). (K–P). Embryos were examined using four probes, ctsl1b (red), dlx3b (blue), ntl (blue), and pcdh8 (red). (O–P) mych MO-induced defects were rescued by co-injection with mych mRNA. lnp, lateral neural plate; mi, midbrain; n, notochord; pp, prechordal plate; r, rhombomere; psm, pre somitic mesoderm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
barhl2 AB control 1-4 somites presumptive diencephalon ISH
AB + MO1-mych standard conditions 1-4 somites presumptive diencephalon ISH
ctslb AB control 1-4 somites prechordal plate ISH
AB + MO1-mych standard conditions 1-4 somites prechordal plate ISH
dlx3b AB control 1-4 somites neural plate ISH
AB + MO1-mych standard conditions 1-4 somites neural plate ISH
dmbx1a AB control 1-4 somites midbrain neural keel ISH
1-4 somites optic primordium ISH
AB + MO1-mych standard conditions 1-4 somites midbrain neural keel ISH
1-4 somites optic primordium ISH
egr2b AB control 1-4 somites presumptive rhombomere 3 ISH
1-4 somites presumptive rhombomere 5 ISH
AB + MO1-mych standard conditions 1-4 somites presumptive rhombomere 3 ISH
1-4 somites presumptive rhombomere 5 ISH
mab21l2 AB control 1-4 somites midbrain neural keel ISH
1-4 somites optic primordium ISH
AB + MO1-mych standard conditions 1-4 somites midbrain neural keel ISH
1-4 somites optic primordium ISH
pax2a AB control 1-4 somites midbrain neural keel ISH
AB + MO1-mych standard conditions 1-4 somites midbrain neural keel ISH
pcdh8 AB control 1-4 somites segmental plate ISH
AB + MO1-mych standard conditions 1-4 somites segmental plate ISH
rx3 AB control 1-4 somites optic primordium ISH
AB + MO1-mych standard conditions 1-4 somites optic primordium ISH
tbxta AB control 1-4 somites notochord ISH
AB + MO1-mych standard conditions 1-4 somites notochord ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-mych standard conditions 1-4 somites eye decreased size, abnormal
1-4 somites hindbrain increased width, abnormal
1-4 somites midbrain increased width, abnormal
1-4 somites trunk increased width, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright.
Open Access
Full text @ PLoS One