ZFIN ID: ZDB-PUB-080501-11
The Mych Gene Is Required for Neural Crest Survival during Zebrafish Development
Hong, S.K., Tsang, M., and Dawid, I.B.
Date: 2008
Source: PLoS One   3(4): e2029 (Journal)
Registered Authors: Dawid, Igor B., Hong, Sung-Kook, Tsang, Michael
Keywords: Embryos, Neural crest, Untranslated regions, Eyes, Zebrafish, Midbrain, Apoptosis, In situ hybridization
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Biomarkers/metabolism
  • Body Patterning/drug effects
  • Brain/drug effects
  • Brain/metabolism
  • Branchial Region/abnormalities
  • Branchial Region/drug effects
  • Cell Death/drug effects
  • DNA, Complementary/isolation & purification
  • Embryo, Nonmammalian/abnormalities
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/metabolism
  • Gene Expression Regulation, Developmental/drug effects
  • Molecular Sequence Data
  • Neural Crest/embryology*
  • Neural Plate/cytology
  • Neural Plate/drug effects
  • Oligonucleotides, Antisense/pharmacology
  • Phenotype
  • Sequence Analysis, Protein
  • Somites/abnormalities
  • Somites/drug effects
  • Transcription Factors/chemistry
  • Transcription Factors/genetics*
  • Transcription Factors/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed: 18446220 Full text @ PLoS One
BACKGROUND: Among Myc family genes, c-Myc is known to have a role in neural crest specification in Xenopus and in craniofacial development in the mouse. There is no information on the function of other Myc genes in neural crest development, or about any developmental role of zebrafish Myc genes.
PRINCIPAL FINDINGS: We isolated the zebrafish mych (myc homologue) gene. Knockdown of mych leads to severe defects in craniofacial development and in certain other tissues including the eye. These phenotypes appear to be caused by cell death in the neural crest and in the eye field in the anterior brain.
SIGNIFICANCE: Mych is a novel factor required for neural crest cell survival in zebrafish.
The corresponding author has contacted ZFIN regarding a correction to a marker name. In Figure 6G and 6H, the expression is attributed to neural crest marker snail1a. In fact, this is a typo and the correct marker name is snai1b.