Multiple defects in pharyngeal arch development caused by mych MO. Control MO (A,C,E,G,I,K) and mych UTR MO-injected embryos (B,D,F,H,J,L). A–B. Lateral view of dlx2a expression in mych UTR MO-injected embryos at 26hpf (B). C–D. Ventral view of expression of hand2 at 36hpf. E–F. Ventral view of tbx1 expression at 45hpf. G–H. Lateral view of fli1-eGFP transgenic line at 40hpf. I–L. Lateral (I,J) and ventral (K,L) views of Alcian blue stained day 5 control (I,K) and mych UTR MO-injected embryos (J,L). ch, ceratohyal, b, branchial arch; e, ethmoid plate; g, gill arches; h, hyoid arch; hs, hyosymplectic; ht, heart; m, mandibular arch; mc, Meckel's cartilage; me, mesoderm; pec, pectoral fin; pq, palatoquadrate.
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