ZFIN ID: ZDB-FIG-080506-2
Hong et al., 2008 - The Mych Gene Is Required for Neural Crest Survival during Zebrafish Development. PLoS One   3(4):e2029 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 8-cell to Protruding-mouth

Fig. 2 Detection of mych transcripts by in situ hybridization.
A. Dorsal view of 8-cell stage embryo. B. Double staining of mych (blue) and gsc (red) at the shield stage; arrowhead points to the shield. C-C′. Lateral (C) and anterior dorsal (C′) views of 80% epiboly stage. Red asterisk points to the anterior-dorsal area shown in C′. D–I. 3-somite stage embryos. D. Dynamic expression of mych in the anterior brain region. E-E′. Double staining with mab21l2 (red) as a marker for eye and midbrain. F-F′. Double staining with dmbx1a (red) as marker for eye field and midbrain; red arrowhead indicates eye field. G,G′. Co-expression with ctsl1b (red), marking the prechordal plate. H-H′. Dorsal view of HuC-positive neuronal cells (red) and mych staining (blue) in wild type (H) and the mibta52b mutant (H′). I. Rhombomeres 3 and 5 are marked by egr2b (red), while mych stains the anterior part of rhombomere 4. J–K. Dorsal views of 10-somite stage embryo showing mych expression in the eye and midbrain (J) and in trunk somites (K). L. Lateral view of mych expression in the brain and pharyngeal system at 24hpf; mandibular (m), hyoid (h), and branchial (b) arches are indicated. M. Expression of mych in the eye at the 36hpf. Arrowhead points to presumptive photoreceptor cell layer. N. mych expression in the heart and intestine (int; magnified in O) at 72hpf. P–Q. Co-expression with foxd3 (red) at the 4-somite stage. The yellow open square area in P is shown as a section in Q. R–S. Pharyngeal arch marker dlx2a (red in S) is co-expressed with mych at 32hpf. b, branchial arch; e, eye; h, hyoid arch; ht, heart; int, intestine; le, lens; m, mandibular arch; mi, midbrain; ov, otic vesicle; pp, prechordal plate; r, rhombomere; s, somite.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ctslb AB standard conditions 1-4 somites prechordal plate ISH
dlx2a AB standard conditions Prim-15 mandibular arch skeleton ISH
Prim-15 pharyngeal arch 2 skeleton ISH
Prim-15 pharyngeal arch 3-7 ISH
dmbx1a AB standard conditions 1-4 somites midbrain neural keel ISH
1-4 somites optic primordium ISH
egr2b AB standard conditions 1-4 somites presumptive rhombomere 3 ISH
1-4 somites presumptive rhombomere 5 ISH
elavl3 AB standard conditions 1-4 somites neuron ISH
mib1ta52b/ta52b standard conditions 1-4 somites neuron ISH
foxd3 AB standard conditions 1-4 somites neural crest ISH
gsc AB standard conditions Shield shield ISH
mab21l2 AB standard conditions 1-4 somites midbrain neural keel ISH
1-4 somites optic primordium ISH
mych AB standard conditions 8-cell whole organism ISH
Shield hypoblast ISH
75%-epiboly prechordal plate ISH
1-4 somites midbrain neural keel ISH
1-4 somites neural crest ISH
1-4 somites neuron ISH
1-4 somites optic primordium ISH
1-4 somites prechordal plate ISH
1-4 somites presumptive rhombomere 4 ISH
10-13 somites immature eye ISH
10-13 somites midbrain ISH
10-13 somites somite ISH
Prim-5 immature eye ISH
Prim-5 mandibular arch skeleton ISH
Prim-5 midbrain ISH
Prim-5 otic vesicle ISH
Prim-5 pharyngeal arch 2 skeleton ISH
Prim-5 pharyngeal arch 3-7 ISH
Prim-15 mandibular arch skeleton ISH
Prim-15 pharyngeal arch 2 skeleton ISH
Prim-15 pharyngeal arch 3-7 ISH
Prim-25 retinal photoreceptor layer ISH
Protruding-mouth heart ISH
Protruding-mouth intestine ISH
Protruding-mouth midbrain ISH
mib1ta52b/ta52b standard conditions 1-4 somites neuron ISH
1-4 somites presumptive rhombomere 4 ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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Open Access
Full text @ PLoS One