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ZFIN ID: ZDB-FIG-080214-9
Dal-Pra et al., 2006 - Noggin1 and Follistatin-like2 function redundantly to Chordin to antagonize BMP activity. Developmental Biology   298(2):514-526 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 75%-epiboly to 1-4 somites

Fig. 6 Analysis of double chordin–noggin1 morphants with endomesodermal markers. (A–C) Expression of draculin (drl) specific of presumptive hematopoietic territory at 70% epiboly. (D–F) Expression of the paraxial marker myf5 at the 3-somite stage. (G–I) Expression of the cephalic mesendodermal marker follistatin1 (fst1) at 80% epiboly. (J–L) Expression of the prechordal plate marker hgg1 which labels the presumptive hatching gland at 75% epiboly. (M–O) Expression of the notochordal marker floating-head (flh) at 75% epiboly. (A, D, G, J, M) Wild-type embryos (B, E, H, K, N), 100 μM MO-chd-injected embryos (C, F, I, L, O) 100–800 μM chd-nog1 MOs-injected embryos. (A–C) Vegetal pole views, dorsal to the right. (D–F) Dorsal views anterior to the left. (G–O) Dorsal views, animal pole up.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ctslb WT standard conditions 75%-epiboly prechordal plate ISH
WT + MO1-chrd standard conditions 75%-epiboly prechordal plate ISH
WT + MO1-chrd + MO1-nog1 standard conditions 75%-epiboly prechordal plate ISH
drl WT standard conditions 75%-epiboly ventral mesoderm ISH
WT + MO1-chrd standard conditions 75%-epiboly ventral mesoderm ISH
WT + MO1-chrd + MO1-nog1 standard conditions 75%-epiboly ventral mesoderm ISH
fsta WT standard conditions 75%-epiboly presumptive cephalic mesoderm ISH
WT + MO1-chrd standard conditions 75%-epiboly presumptive cephalic mesoderm ISH
WT + MO1-chrd + MO1-nog1 standard conditions 75%-epiboly presumptive cephalic mesoderm ISH
myf5 WT standard conditions 1-4 somites segmental plate ISH
WT + MO1-chrd standard conditions 1-4 somites adaxial cell ISH
1-4 somites paraxial mesoderm ISH
WT + MO1-chrd + MO1-nog1 standard conditions 1-4 somites adaxial cell ISH
noto WT standard conditions 75%-epiboly axial chorda mesoderm ISH
WT + MO1-chrd standard conditions 75%-epiboly axial chorda mesoderm ISH
WT + MO1-chrd + MO1-nog1 standard conditions 75%-epiboly axial chorda mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 298(2), Dal-Pra, S., Fürthauer, M., Van-Celst, J., Thisse, B., and Thisse, C., Noggin1 and Follistatin-like2 function redundantly to Chordin to antagonize BMP activity, 514-526, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.