ZFIN ID: ZDB-FIG-080214-7
Dal-Pra et al., 2006 - Noggin1 and Follistatin-like2 function redundantly to Chordin to antagonize BMP activity. Developmental Biology   298(2):514-526 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-15
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-15

Fig. 4 Analysis of double chordin–noggin1 morphants for the expression of tissue-specific molecular markers at 30 hpf. Expression at 30 hpf of three different molecular markers, sox19 labeling the central nervous system, starmaker labeling the otic vesicle and im:7144261 labeling the notochord. (A, E, I, M) Wild-type embryos while the others embryos are injected with MO-chd and MO-nog1 in conditions described in Table 2. (B, F, J, N) Weak aggravation (class I, CI) of the ventralization phenotype, (C, G, K, O) strong ventralization—class II—CII, and (D, H, L, P) extreme ventralization (class 3, CIII). Embryos in lateral view (A–D), (M–P). In dorsal view (E–L), anterior to the left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
im:7144261 WT standard conditions Prim-15 notochord ISH
WT + MO1-chrd standard conditions Prim-15 notochord ISH
WT + MO1-chrd + MO1-nog1 standard conditions Prim-15 notochord ISH
WT + MO1-nog1 standard conditions Prim-15 notochord ISH
sox19a WT standard conditions Prim-15 central nervous system ISH
WT + MO1-chrd standard conditions Prim-15 central nervous system ISH
WT + MO1-chrd + MO1-nog1 standard conditions Prim-15 central nervous system ISH
WT + MO1-nog1 standard conditions Prim-15 central nervous system ISH
stm WT standard conditions Prim-15 otic vesicle ISH
WT + MO1-chrd standard conditions Prim-15 otic vesicle ISH
WT + MO1-chrd + MO1-nog1 standard conditions Prim-15 otic vesicle ISH
WT + MO1-nog1 standard conditions Prim-15 otic vesicle ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-chrd standard conditions Prim-15 neural plate decreased length, abnormal
Prim-15 neural plate decreased thickness, abnormal
Prim-15 otic vesicle decreased size, abnormal
WT + MO1-chrd + MO1-nog1 standard conditions Prim-15 neural plate decreased length, abnormal
Prim-15 neural plate decreased thickness, abnormal
Prim-15 otic vesicle decreased size, abnormal
Prim-15 otic vesicle mislocalised medially, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 298(2), Dal-Pra, S., Fürthauer, M., Van-Celst, J., Thisse, B., and Thisse, C., Noggin1 and Follistatin-like2 function redundantly to Chordin to antagonize BMP activity, 514-526, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.