ZFIN ID: ZDB-FIG-080215-2
Dal-Pra et al., 2006 - Noggin1 and Follistatin-like2 function redundantly to Chordin to antagonize BMP activity. Developmental Biology   298(2):514-526 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 50%-epiboly to Long-pec

Fig. S4 Expression pattern of follistatin-like 2 during embryonic development. (A) Expression of fstl2 starts at the onset of gastrulation in the deep cells of the embryonic shield (sh). (B) At 60% epiboly, fstl2 transcripts are observed in notochord (n) as well as in the yolk syncytial layer (ysl). (C–D) At 75% epiboly and bud stage (E–F), expression is restricted to the notochord. (G–I) At the 7-somite stage, fstl2 expression is observed in notochord as well as in segmental plate (sp) with a stronger staining in the adaxial cells (ad) of the formed somites. Expression is also observed in hindbrain (h). (J) At the 18-somite stage, expression is observed in hindbrain rhombomeres (rh) 2, 4 and 6, somites (s) and in tail epidermis (te). (K–L) At 24 hpf, transcripts accumulate in the choroid fissure (cf), at the rhombomeres boundaries (rhb) in axial vasculature (av) and in posterior somites. (M–N) At 36 hpf, additional expression is observed in forebrain ventricular zone (fv) and epiphysis (ep). (O–P) At 48 hpf, expression is also observed in branchial arches (ba), head mesenchyme (hm) and pectoral fin (pf). Embryos in lateral views anterior to the top in A, B, D and F; in dorsal views anterior to the top in C and E; in lateral views anterior to the left in G and J–O and in dorsal views anterior to the left in H, I and P.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
fstl1b WT standard conditions 50%-epiboly presumptive shield ISH
Shield shield ISH
Shield yolk syncytial layer ISH
75%-epiboly axial chorda mesoderm ISH
Bud axial chorda mesoderm ISH
5-9 somites adaxial cell ISH
5-9 somites hindbrain neural rod ISH
5-9 somites notochord ISH
5-9 somites segmental plate ISH
14-19 somites ectoderm ISH
14-19 somites rhombomere 2 ISH
14-19 somites rhombomere 4 ISH
14-19 somites rhombomere 6 ISH
14-19 somites somite ISH
14-19 somites tail bud ISH
Prim-5 axial vasculature ISH
Prim-5 optic fissure ISH
Prim-5 rhombomere ISH
Prim-5 somite ISH
Prim-25 axial vasculature ISH
Prim-25 epiphysis ISH
Prim-25 rhombomere ISH
Prim-25 ventricular zone ISH
Long-pec head mesenchyme ISH
Long-pec pectoral fin ISH
Long-pec pharyngeal arch 3-7 skeleton ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 298(2), Dal-Pra, S., Fürthauer, M., Van-Celst, J., Thisse, B., and Thisse, C., Noggin1 and Follistatin-like2 function redundantly to Chordin to antagonize BMP activity, 514-526, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.