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ZFIN ID: ZDB-FIG-080214-8
Dal-Pra et al., 2006 - Noggin1 and Follistatin-like2 function redundantly to Chordin to antagonize BMP activity. Developmental Biology   298(2):514-526 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 50%-epiboly to Bud

Fig. 5 Analysis of double chordin–noggin1 morphants for the expression of early molecular markers. Expression of ved (A–C), a Bmp target gene expressed in the ventral part of the gastrula, cyp26a (D–F) expressed at the margin and in anterior neural plate, foxi1 (G–I), a marker of presumptive epidermis at gastrula stage. (A, D, G) Wild type (WT), (B, E, H) injection of 100 μM of MO-chd (class I, CI), (C, F, I) injection of 100–800 μM chd-nog1 MOs (class II, CII). Arrowheads in panels A–C indicate the dorsal limits of ved expression. Arrowheads in panels D–F indicate the anterior limit of the neural plate labeled with cyp26a and arrowheads in G–I, the dorsal limits of the presumptive epidermis labeled with foxi1. Embryos are in animal pole view dorsal to the right in panels A–C and G–I upper panels. Dorsal views animal pole up in panels D–E and G–I lower panels.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cyp26a1 WT standard conditions 50%-epiboly to Bud anterior neural plate ISH
50%-epiboly to Bud margin ISH
WT + MO1-chrd standard conditions 50%-epiboly to Bud anterior neural plate ISH
50%-epiboly to Bud margin ISH
WT + MO1-chrd + MO1-nog1 standard conditions 50%-epiboly to Bud anterior neural plate ISH
50%-epiboly to Bud margin ISH
foxi1 WT standard conditions 50%-epiboly presumptive ectoderm ISH
WT + MO1-chrd standard conditions 50%-epiboly presumptive ectoderm ISH
WT + MO1-chrd + MO1-nog1 standard conditions 50%-epiboly presumptive ectoderm ISH
ved WT standard conditions 50%-epiboly unspecified ISH
WT + MO1-chrd standard conditions 50%-epiboly unspecified ISH
WT + MO1-chrd + MO1-nog1 standard conditions 50%-epiboly unspecified ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 298(2), Dal-Pra, S., Fürthauer, M., Van-Celst, J., Thisse, B., and Thisse, C., Noggin1 and Follistatin-like2 function redundantly to Chordin to antagonize BMP activity, 514-526, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.