ZFIN ID: ZDB-FIG-080304-9
Fgf8 and Fst1 overexpression rescues chordin and chordin–noggin1 morphants. (A) Example of a ventralization phenotype observed after injection of chd or chd-nog1 morpholinos. (B) Example of wild-type like morphology obtained after injection of fgf8 or fst1 RNA into chd or chd-nog1 morphants (C). Example of a dorsalization phenotype observed after injection of fgf8 or fst1 RNA into chd or chd-nog1 morphants. Embryos are observed at 30 hpf in lateral view, anterior to the left. Lower panel: the horizontal bars represent the percentage of embryos displaying a ventralized phenotype (yellow), a wild-type like morphology (green) or a dorsalized phenotype (blue) in chd morphants (MO-chd, 100 μM), double chd-nog1 morphants (MO-chd/MO-nog1—100 μM/800 μM), in chd or chd-nog1 morphants injected with 5 pg of fgf8 RNA (MO-chd + RNA fgf8; MO-chd/MO-nog1 + RNA fgf8, respectively) or with 50 pg of fst1 RNA (MO-chd + RNA fst1; MO-chd/MO-nog1 + RNA fst1, respectively). Due to the lack of two Bmp inhibitors, the rescue of ventralization phenotype (wild-type-like and dorsalized phenotypes) by fgf8 and fst1 is less efficient in double chd-nog1 morphants than in single chd morphants. n: number of embryos analyzed.
Gene Expression DetailsNo data available
Antibody Labeling Details No data available
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article.
Please note that this material may be protected by copyright.
Reprinted from Developmental Biology, 298(2), Dal-Pra, S., Fürthauer, M., Van-Celst, J., Thisse, B., and Thisse, C., Noggin1 and Follistatin-like2 function redundantly to Chordin to antagonize BMP activity, 514-526, Copyright
(2006) with permission from Elsevier.
Full text @ Dev. Biol.