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ZFIN ID: ZDB-FIG-180417-23
Rogers et al., 2017 - Nodal patterning without Lefty inhibitory feedback is functional but fragile. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 30%-epiboly to 75%-epiboly
PHENOTYPE:
Fish:
Observed In:
Stage Range: Sphere to 75%-epiboly

Fig. 3-S1

Expression of nodal, lefty, and Nodal target genes in lefty1-/-;lefty2-/- mutants during gastrulation.

Wild type embryos, lft1-/- embryos, lft2-/- embryos, and (to generate lft1-/-;lft2-/- embryos) progeny from lft1+/-;lft2-/- incrosses, lft1-/-;lft2-/- incrosses, or lft1-/-;lft2-/- X lft1+/-;lft2-/- crosses were fixed at the indicated stages. In situ hybridization was performed using probes against the following Nodal target genes: lft1 (A), lft2 (B), sqt (C), cyc (D), tb/no tail (E), and sox17 (F). Dorsal views. After imaging, embryos from lft1+/-;lft2-/- incrosses or lft1-/-;lft2-/- X lft1+/-;lft2-/- crosses were genotyped (see Materials and methods for details). Upregulation of Nodal target genes was most dramatic in lft1-/-;lft2-/- mutants, though lft1-/- mutants tended to exhibit slight upregulation as well. Note that expression of tb/no tail at 30% epiboly was normal in lft1-/-;lft2-/- mutants, in contrast to the dramatic upregulation at 30% epiboly observed in double lefty morphants (Agathon et al., 2001).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
lft1 WT standard conditions Sphere blastodisc ISH
30%-epiboly margin ISH
Shield margin ISH
50%-epiboly margin ISH
75%-epiboly axis ISH
75%-epiboly margin ISH
lft1a145/a145 standard conditions Sphere blastodisc anatomical margin ISH
30%-epiboly margin ISH
50%-epiboly margin ISH
Shield margin ISH
75%-epiboly axis ISH
75%-epiboly margin ISH
lft1a145/a145 ; lft2a146/a146 standard conditions Sphere blastodisc anatomical margin ISH
30%-epiboly margin ISH
50%-epiboly margin ISH
Shield margin ISH
75%-epiboly axis ISH
75%-epiboly margin ISH
lft2a146/a146 standard conditions Sphere blastodisc ISH
30%-epiboly margin ISH
Shield margin ISH
50%-epiboly margin ISH
75%-epiboly axis ISH
75%-epiboly margin ISH
lft2 WT standard conditions Sphere blastodisc ISH
50%-epiboly margin ISH
30%-epiboly margin ISH
Shield margin ISH
75%-epiboly axis ISH
lft1a145/a145 standard conditions Sphere blastodisc ISH
30%-epiboly margin ISH
Shield margin ISH
50%-epiboly margin ISH
75%-epiboly axis ISH
lft1a145/a145 ; lft2a146/a146 standard conditions Sphere blastodisc ISH
30%-epiboly margin ISH
Shield margin ISH
50%-epiboly margin ISH
75%-epiboly axis ISH
lft2a146/a146 standard conditions Sphere blastodisc ISH
50%-epiboly margin ISH
30%-epiboly margin ISH
Shield margin ISH
75%-epiboly axis ISH
ndr1 WT standard conditions Sphere blastodisc ISH
50%-epiboly margin ISH
30%-epiboly margin ISH
Shield margin ISH
75%-epiboly margin ISH
lft1a145/a145 standard conditions Sphere blastodisc ISH
50%-epiboly margin ISH
30%-epiboly margin ISH
Shield margin ISH
75%-epiboly margin ISH
lft1a145/a145 ; lft2a146/a146 standard conditions Sphere blastodisc ISH
50%-epiboly margin ISH
30%-epiboly margin ISH
Shield margin ISH
75%-epiboly margin ISH
lft2a146/a146 standard conditions Sphere blastodisc ISH
30%-epiboly margin ISH
Shield margin ISH
50%-epiboly margin ISH
ndr2 WT standard conditions Sphere blastodisc ISH
50%-epiboly margin ISH
30%-epiboly margin ISH
75%-epiboly axis ISH
lft1a145/a145 standard conditions Sphere blastodisc ISH
30%-epiboly margin ISH
50%-epiboly margin ISH
75%-epiboly axis ISH
lft1a145/a145 ; lft2a146/a146 standard conditions Sphere blastodisc ISH
30%-epiboly margin ISH
50%-epiboly margin ISH
75%-epiboly axis ISH
lft2a146/a146 standard conditions Sphere blastodisc ISH
30%-epiboly margin ISH
50%-epiboly margin ISH
75%-epiboly axis ISH
sox17 WT standard conditions Sphere blastodisc ISH
Shield margin ISH
50%-epiboly margin ISH
30%-epiboly margin ISH
75%-epiboly endoderm ISH
lft1a145/a145 standard conditions Sphere blastodisc ISH
50%-epiboly margin ISH
30%-epiboly margin ISH
Shield margin ISH
75%-epiboly endoderm ISH
lft1a145/a145 ; lft2a146/a146 standard conditions 30%-epiboly margin ISH
50%-epiboly margin ISH
Shield margin ISH
75%-epiboly endoderm ISH
lft2a146/a146 standard conditions Sphere blastodisc ISH
50%-epiboly margin ISH
30%-epiboly margin ISH
Shield margin ISH
75%-epiboly endoderm ISH
tbxta WT standard conditions Sphere blastodisc ISH
50%-epiboly margin ISH
30%-epiboly margin ISH
Shield margin ISH
75%-epiboly margin ISH
lft1a145/a145 standard conditions Sphere blastodisc ISH
50%-epiboly margin ISH
30%-epiboly margin ISH
Shield margin ISH
75%-epiboly margin ISH
lft1a145/a145 ; lft2a146/a146 standard conditions Sphere blastodisc ISH
50%-epiboly margin ISH
30%-epiboly margin ISH
Shield margin ISH
75%-epiboly margin ISH
lft2a146/a146 standard conditions Sphere blastodisc ISH
30%-epiboly margin ISH
Shield margin ISH
50%-epiboly margin ISH
75%-epiboly margin ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
lft1a145/a145 standard conditions Sphere blastodisc anatomical margin lft1 expression increased amount, abnormal
30%-epiboly margin ndr1 expression increased amount, abnormal
Shield margin lft1 expression increased amount, abnormal
Shield margin ndr1 expression increased amount, abnormal
50%-epiboly margin ndr1 expression increased amount, abnormal
50%-epiboly margin lft1 expression increased amount, abnormal
30%-epiboly margin lft1 expression increased amount, abnormal
Shield margin lft1 expression increased distribution, abnormal
Shield margin ndr1 expression increased distribution, abnormal
50%-epiboly margin lft1 expression increased distribution, abnormal
50%-epiboly margin ndr1 expression increased distribution, abnormal
30%-epiboly margin ndr1 expression increased distribution, abnormal
30%-epiboly margin lft1 expression increased distribution, abnormal
75%-epiboly axis ndr2 expression increased amount, abnormal
75%-epiboly axis lft1 expression increased amount, abnormal
75%-epiboly axis lft1 expression increased distribution, abnormal
75%-epiboly axis ndr2 expression increased distribution, abnormal
75%-epiboly axis lft1 expression spatial pattern, abnormal
75%-epiboly margin lft1 expression increased amount, abnormal
75%-epiboly margin lft1 expression increased distribution, abnormal
75%-epiboly margin lft1 expression spatial pattern, abnormal
lft1a145/a145 ; lft2a146/a146 standard conditions Sphere blastodisc anatomical margin lft1 expression increased amount, abnormal
30%-epiboly margin ndr1 expression increased amount, abnormal
30%-epiboly margin lft2 expression increased amount, abnormal
30%-epiboly margin ndr1 expression increased distribution, abnormal
30%-epiboly margin lft2 expression increased distribution, abnormal
30%-epiboly margin lft1 expression increased distribution, abnormal
Shield margin lft2 expression increased amount, abnormal
Shield margin ndr1 expression increased amount, abnormal
Shield margin lft1 expression increased amount, abnormal
50%-epiboly margin lft2 expression increased amount, abnormal
50%-epiboly margin ndr1 expression increased amount, abnormal
50%-epiboly margin lft1 expression increased amount, abnormal
30%-epiboly margin lft1 expression increased amount, abnormal
Shield margin ndr1 expression increased distribution, abnormal
Shield margin lft1 expression increased distribution, abnormal
Shield margin lft2 expression increased distribution, abnormal
50%-epiboly margin lft2 expression increased distribution, abnormal
50%-epiboly margin ndr1 expression increased distribution, abnormal
50%-epiboly margin lft1 expression increased distribution, abnormal
Shield margin lft2 expression spatial pattern, abnormal
50%-epiboly margin lft2 expression spatial pattern, abnormal
30%-epiboly margin lft2 expression spatial pattern, abnormal
75%-epiboly axis lft2 expression increased amount, abnormal
75%-epiboly axis ndr2 expression increased amount, abnormal
75%-epiboly axis lft1 expression increased amount, abnormal
75%-epiboly axis lft2 expression increased distribution, abnormal
75%-epiboly axis ndr2 expression increased distribution, abnormal
75%-epiboly axis lft1 expression increased distribution, abnormal
75%-epiboly axis lft2 expression spatial pattern, abnormal
75%-epiboly axis lft1 expression spatial pattern, abnormal
75%-epiboly endoderm sox17 expression increased amount, abnormal
75%-epiboly endoderm sox17 expression increased distribution, abnormal
75%-epiboly endoderm sox17 expression spatial pattern, abnormal
75%-epiboly margin lft1 expression increased amount, abnormal
75%-epiboly margin lft1 expression increased distribution, abnormal
75%-epiboly margin lft1 expression spatial pattern, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife