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ZFIN ID: ZDB-FIG-180417-22
Rogers et al., 2017 - Nodal patterning without Lefty inhibitory feedback is functional but fragile. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Sphere to 75%-epiboly
PHENOTYPE:
Fish:
Observed In:
Stage Range: Shield to 75%-epiboly

Fig. 3

Lefty loss causes expanded expression of mesendodermal genes.

In situ hybridization comparing expression of endodermal (sox32/casanova) (A–E’’’) and mesodermal (noto/floating head) (F–J’’’) genes in wild type, lft1-/- mutants, lft2-/- mutants, and lft1-/-;lft2-/- mutants at the indicated developmental stages (dorsal views). Note upregulation of mesendoderm in lft1-/-;lft2-/- mutants starting at early gastrulation stages (e.g., shield stage). lft1-/-;lft2-/- embryos were generated from lft1+/-;lft2-/- incrosses, lft1-/-;lft2-/- incrosses, or lft1-/-;lft2-/- X lft1+/-;lft2-/- crosses. Embryos were genotyped after imaging (see Materials and methods for details).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
noto WT standard conditions Sphere presumptive mesoderm ISH
50%-epiboly presumptive mesoderm ISH
30%-epiboly presumptive mesoderm ISH
Shield presumptive mesoderm ISH
75%-epiboly mesoderm ISH
lft1a145/a145 standard conditions 50%-epiboly presumptive mesoderm ISH
30%-epiboly presumptive mesoderm ISH
Sphere presumptive mesoderm ISH
Shield presumptive mesoderm ISH
75%-epiboly mesoderm ISH
lft1a145/a145 ; lft2a146/a146 standard conditions 50%-epiboly presumptive mesoderm ISH
30%-epiboly presumptive mesoderm ISH
Sphere presumptive mesoderm ISH
Shield presumptive mesoderm ISH
75%-epiboly mesoderm ISH
lft2a146/a146 standard conditions Sphere presumptive mesoderm ISH
50%-epiboly presumptive mesoderm ISH
30%-epiboly presumptive mesoderm ISH
Shield presumptive mesoderm ISH
75%-epiboly mesoderm ISH
sox32 WT standard conditions 50%-epiboly presumptive endoderm ISH
30%-epiboly presumptive endoderm ISH
Sphere presumptive endoderm ISH
Shield presumptive endoderm ISH
75%-epiboly endoderm ISH
lft1a145/a145 standard conditions Sphere presumptive endoderm ISH
30%-epiboly presumptive endoderm ISH
Shield presumptive endoderm ISH
50%-epiboly presumptive endoderm ISH
75%-epiboly endoderm ISH
lft1a145/a145 ; lft2a146/a146 standard conditions Sphere presumptive endoderm ISH
50%-epiboly presumptive endoderm ISH
30%-epiboly presumptive endoderm ISH
Shield presumptive endoderm ISH
75%-epiboly endoderm ISH
lft2a146/a146 standard conditions 50%-epiboly presumptive endoderm ISH
30%-epiboly presumptive endoderm ISH
Sphere presumptive endoderm ISH
Shield presumptive endoderm ISH
75%-epiboly endoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
lft1a145/a145 ; lft2a146/a146 standard conditions Shield presumptive endoderm sox32 expression increased amount, abnormal
Shield presumptive endoderm sox32 expression increased distribution, abnormal
Shield presumptive endoderm sox32 expression spatial pattern, abnormal
Shield presumptive mesoderm noto expression increased amount, abnormal
Shield presumptive mesoderm noto expression increased distribution, abnormal
Shield presumptive mesoderm noto expression spatial pattern, abnormal
75%-epiboly endoderm sox32 expression increased amount, abnormal
75%-epiboly endoderm sox32 expression increased distribution, abnormal
75%-epiboly endoderm sox32 expression spatial pattern, abnormal
75%-epiboly mesoderm noto expression increased amount, abnormal
75%-epiboly mesoderm noto expression increased distribution, abnormal
75%-epiboly mesoderm noto expression spatial pattern, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife