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ZFIN ID: ZDB-FIG-180417-30
Rogers et al., 2017 - Nodal patterning without Lefty inhibitory feedback is functional but fragile. eLIFE   6 Full text @ Elife
Observed In:
Stage: Prim-5

Fig. 6-S1

Artificial, uniform Nodal inhibition rescues morphology at 1 dpf in lefty1-/-;lefty2-/- mutants.

Wild type and lft1-/-;lft2-/- mutant embryos were exposed to 0, 1.25, 2.5, 3.75, 5, or 6.25 μM SB-505124 (Nodal inhibitor drug) starting at the 8 cell stage, sphere stage, or shield stage. Drug was removed at 24 hr post-fertilization. Rescue at earlier stages requires lower concentrations (2.5–3.75 μM) of SB-505124 than rescue at later stages (5–6.25 μM). (A–B) Unexposed wild type (A) and lft1-/-;lft2-/- mutant (B) embryos at 1 dpf. (C–Q’) Embryos exposed to SB-505124 from the 8 cell stage to 24 hpf (C–G’), sphere stage to 24 hpf (H–L’), and shield stage to 24 hpf (M–Q’) at 1 dpf. Number of embryos with same phenotype as image/total is indicated in bottom right of images. Cyclopia and loss of head and trunk mesendoderm are typical Nodal loss-of-function phenotypes (Gritsman et al., 1999; Hagos and Dougan, 2007), and can be observed in both wild type and mutant embryos treated with higher amounts of SB-505124 (e.g., F and F’). Wild type embryos exposed to 3.75 μM at the 8 cell stage (E) exhibited cyclopia (15/27, shown here) and normal morphology (12/27). Wild type embryos exposed to 3.75 μM at sphere stage (J) exhibited mild cyclopia (6/30), moderate cyclopia (13/30, shown here), necrosis and cyclopia (1/30), normal morphology (9/30), and 1/30 died. Some images shown here are also used in Figure 6.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment by environment: SB 505124 Prim-5 eye fused with eye, abnormal
lft1a145/a145 ; lft2a146/a146 standard conditions Prim-5 caudal fin curved dorsal, abnormal
Prim-5 caudal fin decreased length, abnormal
Prim-5 caudal fin morphology, abnormal
Prim-5 eye aplastic, abnormal
Prim-5 eye morphology, abnormal
Prim-5 whole organism decreased length, abnormal
Prim-5 whole organism morphology, abnormal
lft1a145/a145 ; lft2a146/a146 chemical treatment by environment: SB 505124 Prim-5 caudal fin length, ameliorated
Prim-5 caudal fin morphology, ameliorated
Prim-5 eye morphology, ameliorated
Prim-5 whole organism morphology, ameliorated
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife