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ZFIN ID: ZDB-FIG-180417-31
Rogers et al., 2017 - Nodal patterning without Lefty inhibitory feedback is functional but fragile. eLIFE   6 Full text @ Elife
Observed In:
Stage Range: Day 4 to Adult

Fig. 6-S2

Artificial, uniform Nodal inhibition rescues morphology at 4 dpf in lefty1-/-;lefty2-/- mutants.

(A) Wild type and lft1-/-;lft2-/- mutant embryos were exposed to 0, 1.25, 2.5, 3.75, 5, or 6.25 μM SB-505124 (Nodal inhibitor drug) starting at the 8 cell stage, sphere stage, or shield stage. Drug was removed at 24 hr post-fertilization. Percent of embryos with normal gross morphology at 4 days post-fertilization (dpf) is plotted. (B) Unexposed wild type embryo at 4 dpf. (C) A ~ 3 month old female lft1-/-;lft2-/- mutant exposed to 2.5 μM SB-505124 from the 8 cell stage to 24 hpf. Drug-rescued mutants are fertile. Spotted pigmentation is common in incrossed TLAB strains. (D–R’) Embryos exposed to SB-505124 from the 8 cell stage to 24 hpf (D–H’), sphere stage to 24 hpf (I–M’), and shield stage to 24 hpf (N–R’) at 4 dpf. Number of embryos with same phenotype as image out of total is indicated in bottom right of images. Occasionally, embryos that appeared normal at 1 dpf had evident defects at 4 dpf, and embryos that appeared abnormal at 1 dpf recovered by 4 dpf.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment by environment: SB 505124 Day 4 axis curved, abnormal
Day 4 whole organism decreased length, abnormal
Day 4 whole organism morphology, abnormal
lft1a145/a145 ; lft2a146/a146 chemical treatment by environment: SB 505124 Day 4 caudal fin length, ameliorated
Day 4 caudal fin morphology, ameliorated
Day 4 eye morphology, ameliorated
Adult whole organism fertile, normal
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife