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ZFIN ID: ZDB-FIG-180417-31
Rogers et al., 2017 - Nodal patterning without Lefty inhibitory feedback is functional but fragile. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Day 4 to Adult

Fig. 6-S2

Artificial, uniform Nodal inhibition rescues morphology at 4 dpf in lefty1-/-;lefty2-/- mutants.

(A) Wild type and lft1-/-;lft2-/- mutant embryos were exposed to 0, 1.25, 2.5, 3.75, 5, or 6.25 μM SB-505124 (Nodal inhibitor drug) starting at the 8 cell stage, sphere stage, or shield stage. Drug was removed at 24 hr post-fertilization. Percent of embryos with normal gross morphology at 4 days post-fertilization (dpf) is plotted. (B) Unexposed wild type embryo at 4 dpf. (C) A ~ 3 month old female lft1-/-;lft2-/- mutant exposed to 2.5 μM SB-505124 from the 8 cell stage to 24 hpf. Drug-rescued mutants are fertile. Spotted pigmentation is common in incrossed TLAB strains. (D–R’) Embryos exposed to SB-505124 from the 8 cell stage to 24 hpf (D–H’), sphere stage to 24 hpf (I–M’), and shield stage to 24 hpf (N–R’) at 4 dpf. Number of embryos with same phenotype as image out of total is indicated in bottom right of images. Occasionally, embryos that appeared normal at 1 dpf had evident defects at 4 dpf, and embryos that appeared abnormal at 1 dpf recovered by 4 dpf.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment by environment: SB 505124 Day 4 axis curved, abnormal
Day 4 whole organism decreased length, abnormal
Day 4 whole organism morphology, abnormal
lft1a145/a145 ; lft2a146/a146 chemical treatment by environment: SB 505124 Day 4 caudal fin length, ameliorated
Day 4 caudal fin morphology, ameliorated
Day 4 eye morphology, ameliorated
Adult whole organism fertile, normal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife