ZFIN ID: ZDB-FIG-180417-24
Rogers et al., 2017 - Nodal patterning without Lefty inhibitory feedback is functional but fragile. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 30%-epiboly to 75%-epiboly
PHENOTYPE:
Fish:
Observed In:
Stage Range: 30%-epiboly to Prim-5

Fig. 4

nodal mutations suppress lefty1-/-;lefty2-/- mutant defects.

(A–F’’) Lateral views of embryos with indicated genotypes at 1 day post-fertilization (dpf). Embryos are progeny from an incross of cycm294/+;lft1-/-;lft2+/- (A–C’’) or sqtcz35/+;lft1-/-;lft2+/- (D–F’’) adults, and were genotyped after imaging (see Materials and methods for details). In contrast to lft1-/-;lft2-/- mutants (A’’, D’’), nodal+/-;lft1-/-;lft2-/- mutants (B’’, E’’) have long tails and well-defined heads, and some nodal-/-;lft1-/-;lft2-/- mutants (C’’,F’’) have eyes in addition to full-length tails. nodal-/-;lft1-/-;lft2-/- and nodal+/-;lft1-/-;lft2-/- mutants are not viable. cyc homozygotes exhibit the expected curved body axis (Sampath et al., 1998) (C,C’), but curvature is reduced in cyc-/-;lft1-/-;lft2-/- embryos (C’’). (G–L’’’) In situ hybridization assessing expression of endodermal (sox32/casanova) (G–I’’’) or mesodermal (noto/floating head) (J–L’’’) genes in the indicated genotypes (dorsal views). Mesendoderm upregulation is less pronounced in sqt-/-;lft1-/-;lft2-/- compared to lft1-/-;lft2-/- mutants. (M–N) 72 embryos from a cyc+/-;lft1-/-;lft2+/- incross (M) and 84 embryos from a sqt+/-;lft1-/-;lft2+/- incross (N) were scored and imaged at 1 dpf, and subsequently genotyped. Number of embryos of each genotype with the indicated phenotype at 1 dpf is shown. Together, these results demonstrate that loss of sqt or cyc can suppress lft1-/-;lft2-/- mutant phenotypes.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
noto WT standard conditions 30%-epiboly margin ISH
Shield margin ISH
75%-epiboly axis ISH
lft1a145/a145 ; lft2a146/a146 standard conditions 30%-epiboly margin ISH
Shield margin ISH
75%-epiboly axis ISH
lft1a145/a145 ; lft2a146/a146 ; ndr1cz35/cz35 standard conditions 30%-epiboly margin ISH
Shield margin ISH
75%-epiboly axis ISH
ndr1cz35/cz35 standard conditions 30%-epiboly margin ISH
Shield margin ISH
75%-epiboly axis ISH
sox32 WT standard conditions 30%-epiboly margin ISH
Shield margin ISH
75%-epiboly endoderm ISH
lft1a145/a145 ; lft2a146/a146 standard conditions 30%-epiboly margin ISH
Shield margin ISH
75%-epiboly endoderm ISH
lft1a145/a145 ; lft2a146/a146 ; ndr1cz35/cz35 standard conditions 30%-epiboly margin ISH
Shield margin ISH
75%-epiboly endoderm ISH
ndr1cz35/cz35 standard conditions 30%-epiboly margin ISH
Shield margin ISH
75%-epiboly endoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
lft1a145/a145 ; lft2a146/a146 standard conditions Shield margin sox32 expression increased amount, abnormal
Shield margin sox32 expression increased distribution, abnormal
Shield margin sox32 expression spatial pattern, abnormal
75%-epiboly axis noto expression increased amount, abnormal
75%-epiboly axis noto expression increased distribution, abnormal
75%-epiboly axis noto expression spatial pattern, abnormal
75%-epiboly endoderm sox32 expression increased amount, abnormal
75%-epiboly endoderm sox32 expression increased distribution, abnormal
75%-epiboly endoderm sox32 expression spatial pattern, abnormal
lft1a145/a145 ; lft2a146/a146 ; ndr1cz35/cz35 standard conditions 30%-epiboly margin noto expression decreased amount, abnormal
30%-epiboly margin noto expression decreased distribution, abnormal
30%-epiboly margin noto expression spatial pattern, abnormal
Shield margin noto expression decreased amount, abnormal
Shield margin noto expression decreased distribution, abnormal
Shield margin noto expression spatial pattern, abnormal
75%-epiboly axis noto expression decreased amount, abnormal
75%-epiboly axis noto expression decreased distribution, abnormal
75%-epiboly axis noto expression spatial pattern, abnormal
Prim-5 axis bifurcated, abnormal
lft1a145/a145 ; lft2a146/a146 ; ndr2m294/m294 standard conditions Prim-5 caudal fin length, normal
Prim-5 eye morphology, normal
lft1a145/a145 ; ndr1cz35/+ ; lft2a146/+ standard conditions Prim-5 axis curved, abnormal
lft2a146/+ ; ndr1cz35/cz35 ; lft1a145/a145 standard conditions Prim-5 axis curved, abnormal
lft2a146/a146 ; lft1a145/a145 ; ndr1cz35/+ standard conditions Prim-5 caudal fin curved dorsal, abnormal
Prim-5 eye aplastic, abnormal
Prim-5 eye morphology, normal
Prim-5 eye morphology, abnormal
Prim-5 notochord kinked, abnormal
Prim-5 whole organism decreased length, abnormal
Prim-5 whole organism morphology, abnormal
ndr1cz35/cz35 standard conditions 30%-epiboly margin noto expression decreased amount, abnormal
30%-epiboly margin noto expression decreased distribution, abnormal
30%-epiboly margin noto expression spatial pattern, abnormal
Shield margin noto expression decreased amount, abnormal
Shield margin noto expression decreased distribution, abnormal
Shield margin noto expression spatial pattern, abnormal
75%-epiboly axis noto expression decreased amount, abnormal
75%-epiboly axis noto expression decreased distribution, abnormal
75%-epiboly axis noto expression spatial pattern, abnormal
ndr2m294/+ ; lft1a145/a145 ; lft2a146/+ standard conditions Prim-5 eye morphology, abnormal
Prim-5 notochord kinked, abnormal
ndr2m294/+ ; lft1a145/a145 ; lft2a146/a146 standard conditions Prim-5 caudal fin decreased length, abnormal
Prim-5 caudal fin morphology, abnormal
Prim-5 eye aplastic, abnormal
Prim-5 eye morphology, abnormal
Prim-5 notochord kinked, abnormal
Prim-5 whole organism decreased length, abnormal
Prim-5 whole organism morphology, abnormal
ndr2m294/m294 ; lft2a146/+ ; lft1a145/a145 standard conditions Prim-5 caudal fin length, normal
Prim-5 eye morphology, normal
Prim-5 notochord kinked, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife