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ZIRC
ZFIN ID: ZDB-FIG-180417-18
Rogers et al., 2017 - Nodal patterning without Lefty inhibitory feedback is functional but fragile. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Prim-15

Fig. 1-S1

lefty1-/- mutants exhibit partially penetrant heart laterality defects.

(A) In situ hybridization using a probe against the heart muscle marker myl7 in 24 hr post-fertilization (hpf) wild type and lft mutant embryos (dorsal views). Most wild type and lft2-/- mutants exhibit myl7 expression on the left side. In contrast, some lft1-/- mutants exhibit bilateral myl7 expression, and lft1-/-;lft2-/- mutants express very little myl7 and typically fail to generate hearts (dorsal views). (B) Quantification of heart laterality defects in lft1-/- and lft2-/- mutants (ventral views). Live embryos were scored at 30 hpf. Whereas lft2-/- mutants typically exhibit normal heart laterality, lft1-/- mutants frequently have misplaced hearts. Despite this heart laterality defect, lft1-/- mutants are viable.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
myl7 WT standard conditions Prim-5 heart tube ISH
lft1a145/a145 standard conditions Prim-5 heart tube ISH
lft1a145/a145 ; lft2a146/a146 standard conditions Prim-5 Not Detected heart tube ISH
lft2a146/a146 standard conditions Prim-5 heart tube ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
lft1a145/a145 standard conditions Prim-5 heart tube bifurcated, abnormal
Prim-5 heart tube myl7 expression spatial pattern, abnormal
Prim-15 heart mislocalised laterally, abnormal
Prim-15 heart mislocalised medially, abnormal
Prim-15 whole organism viable, normal
lft1a145/a145 ; lft2a146/a146 standard conditions Prim-5 heart tube myl7 expression absent, abnormal
Prim-5 heart tube aplastic, abnormal
lft2a146/a146 standard conditions Prim-15 heart position, normal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife