header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-180417-32
Rogers et al., 2017 - Nodal patterning without Lefty inhibitory feedback is functional but fragile. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: 30%-epiboly to 75%-epiboly
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: 30%-epiboly to 75%-epiboly

Fig. 6-S3

Artificial, uniform Nodal inhibition rescues mesendodermal gene expression in lefty1-/-;lefty2-/- mutants.

Wild type and lft1-/-;lft2-/- mutant embryos were exposed to 2.5 μM SB-505124 (Nodal inhibitor drug) starting at the 8 cell stage (A,B), 3 μM starting at sphere stage (C,D), or 5 μM starting at shield stage (E,F), slightly after gastrulation commences. Endodermal (sox32/casanova) (A,C,E) and mesodermal (noto/floating head) (B,D,F) gene expression was assessed using in situ hybridization. Embryos were fixed at the indicated times post drug-exposure. Mesodermal gene expression in lft1-/-;lft2-/- mutants exposed at the 8 cell stage or sphere (B,D) appears normal, while endodermal gene expression in drug-exposed embryos is reduced (A,C). In contrast, mesendodermal gene expression is upregulated in embryos exposed at shield stage until ~5 hr post-exposure (E,F). Some images from A, B, E, and F are also used in Figure 6.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
noto WT standard conditions 30%-epiboly margin ISH
Shield margin ISH
75%-epiboly axis ISH
WT chemical treatment by environment: SB 505124 30%-epiboly margin ISH
Shield margin ISH
75%-epiboly axis ISH
lft1a145/a145 ; lft2a146/a146 standard conditions 30%-epiboly margin ISH
Shield margin ISH
75%-epiboly axis ISH
lft1a145/a145 ; lft2a146/a146 chemical treatment by environment: SB 505124 30%-epiboly margin ISH
Shield margin ISH
75%-epiboly axis ISH
sox32 WT standard conditions 30%-epiboly margin ISH
Shield margin ISH
75%-epiboly endoderm ISH
WT chemical treatment by environment: SB 505124 30%-epiboly margin ISH
Shield margin ISH
75%-epiboly endoderm ISH
lft1a145/a145 ; lft2a146/a146 standard conditions 30%-epiboly margin ISH
Shield margin ISH
75%-epiboly endoderm ISH
lft1a145/a145 ; lft2a146/a146 chemical treatment by environment: SB 505124 30%-epiboly margin ISH
Shield margin ISH
75%-epiboly endoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment by environment: SB 505124 30%-epiboly margin sox32 expression decreased amount, abnormal
Shield margin sox32 expression decreased amount, abnormal
Shield margin noto expression decreased amount, abnormal
lft1a145/a145 ; lft2a146/a146 standard conditions 30%-epiboly margin noto expression decreased amount, abnormal
Shield margin sox32 expression increased distribution, abnormal
Shield margin sox32 expression spatial pattern, abnormal
75%-epiboly axis noto expression increased amount, abnormal
75%-epiboly axis noto expression increased distribution, abnormal
75%-epiboly axis noto expression spatial pattern, abnormal
75%-epiboly endoderm sox32 expression increased amount, abnormal
75%-epiboly endoderm sox32 expression increased distribution, abnormal
75%-epiboly endoderm sox32 expression spatial pattern, abnormal
lft1a145/a145 ; lft2a146/a146 chemical treatment by environment: SB 505124 30%-epiboly margin sox32 expression decreased amount, abnormal
75%-epiboly axis noto expression amount, ameliorated
75%-epiboly axis noto expression spatial pattern, ameliorated
75%-epiboly endoderm sox32 expression amount, ameliorated
75%-epiboly endoderm sox32 expression spatial pattern, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife