ZFIN ID: ZDB-FIG-160505-9
Han et al., 2016 - Trim69 regulates zebrafish brain development by ap-1 pathway. Scientific Reports   6:24034 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 3

Trim69 knockdown affects zebrafish embryo neurogenesis.

(A) The expression of neuronal differentiation markers was decreased in response to trim69 knockdown. trim69-CON: control group; trim69-MO: trim69 knocking down; black arrow indicates positive signals; scale bar: 200 µm; (B) The expression of stem cell markers was decreased in response to trim69 knockdown. trim69-CON: control group; trim69-MO: trim69 knocking down; black arrow indicates positive signals; scale bar: 200 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ascl1a WT control Prim-5 brain ISH
WT + MO1-trim69 standard conditions Prim-5 brain ISH
gfap WT control Prim-5 brain ISH
WT + MO1-trim69 standard conditions Prim-5 brain ISH
nanog WT control Prim-5 brain ISH
Prim-5 neuronal stem cell ISH
WT + MO1-trim69 standard conditions Prim-5 brain ISH
Prim-5 neuronal stem cell ISH
pax2a WT control Prim-5 brain ISH
WT + MO1-trim69 standard conditions Prim-5 brain ISH
pax3a WT control Prim-5 brain ISH
WT + MO1-trim69 standard conditions Prim-5 brain ISH
pax6a WT control Prim-5 brain ISH
WT + MO1-trim69 standard conditions Prim-5 brain ISH
pax7a WT control Prim-5 brain ISH
WT + MO1-trim69 standard conditions Prim-5 brain ISH
sox2 WT control Prim-5 brain ISH
Prim-5 neuronal stem cell ISH
WT + MO1-trim69 standard conditions Prim-5 brain ISH
Prim-5 neuronal stem cell ISH
wnt1 WT control Prim-5 brain ISH
WT + MO1-trim69 standard conditions Prim-5 brain ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-trim69 standard conditions Prim-5 brain nanog expression decreased amount, abnormal
WT + MO1-trim69 standard conditions Prim-5 brain gfap expression decreased amount, abnormal
WT + MO1-trim69 standard conditions Prim-5 brain wnt1 expression decreased amount, abnormal
WT + MO1-trim69 standard conditions Prim-5 brain pax7a expression decreased amount, abnormal
WT + MO1-trim69 standard conditions Prim-5 brain pax6a expression decreased amount, abnormal
WT + MO1-trim69 standard conditions Prim-5 brain pax2a expression decreased amount, abnormal
WT + MO1-trim69 standard conditions Prim-5 brain ascl1a expression decreased amount, abnormal
WT + MO1-trim69 standard conditions Prim-5 brain pax3a expression decreased amount, abnormal
WT + MO1-trim69 standard conditions Prim-5 brain sox2 expression decreased amount, abnormal
WT + MO1-trim69 standard conditions Prim-5 neuronal stem cell nanog expression decreased amount, abnormal
WT + MO1-trim69 standard conditions Prim-5 neuronal stem cell sox2 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.