ZFIN ID: ZDB-FIG-160505-11
Han et al., 2016 - Trim69 regulates zebrafish brain development by ap-1 pathway. Scientific Reports   6:24034 Full text @ Sci. Rep.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 7

Loss of c-Jun rescued the deformed brain and apoptosis induced by trim69 knockdown.

(A) Loss of c-Jun rescued the deformed brain induced by trim69 knockdown. trim69-CON(a): control group; trim69-MO(b): trim69 knocking down; trim69-MO:c-Jun-MO(c): co-injection with trim69-MO and c-Jun-MO; black arrow indicates mid-hind brain boundary(MHB); scale bar: 200 µm; (d) Statistical analysis of the zebrafish embryo phenotype. Normal: the phenotype of the zebrafish embryo is normal; deformed: the phenotype of the zebrafish embryo phenotype is abnormal. (B) The examined apoptosis of zebrafish embryos by TUNEL assay. trim69-CON(e): control group; trim69-MO(f): trim69 knocking down; trim69-MO:cJun-MO(g): co-injection with trim69-MO and c-Jun-MO; black arrow indicates apoptotic cells; scale bar: 200 µm.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-trim69 standard conditions Prim-5 brain apoptotic, abnormal
Prim-5 brain deformed, abnormal
Prim-5 brain development disrupted, abnormal
Prim-5 midbrain hindbrain boundary absent, abnormal
WT + MO1-trim69 + MO2-jun standard conditions Prim-5 apoptotic process process quality, ameliorated
Prim-5 brain morphology, ameliorated
Prim-5 brain development process quality, ameliorated
Prim-5 midbrain hindbrain boundary morphology, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.