ZFIN ID: ZDB-FIG-160505-12
Han et al., 2016 - Trim69 regulates zebrafish brain development by ap-1 pathway. Scientific Reports   6:24034 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 8

Loss of c-Jun rescued the zebrafish embryo neurogenesis.

(A) Loss of c-Jun rescued the expression of neuronal differentiation markers. trim69-MO(f): trim69 knocking down; trim69-MO:cJun-MO(g): co-injection with trim69-MO and c-Jun-MO; black arrow indicates positive signals; scale bar: 200 µm; (B) Loss of c-Jun rescued the expression of stem cell markers. trim69-MO(f): trim69 knocking down; trim69-MO:cJun-MO(g): co-injection with trim69-MO and c-Jun-MO; black arrow indicates positive signals; scale bar: 200 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ascl1a WT control Prim-5 brain ISH
WT + MO1-trim69 + MO2-jun standard conditions Prim-5 brain ISH
nanog WT control Prim-5 brain ISH
WT + MO1-trim69 + MO2-jun standard conditions Prim-5 brain ISH
pax3a WT control Prim-5 brain ISH
WT + MO1-trim69 + MO2-jun standard conditions Prim-5 brain ISH
pax6a WT control Prim-5 brain ISH
WT + MO1-trim69 + MO2-jun standard conditions Prim-5 brain ISH
pax7a WT control Prim-5 brain ISH
WT + MO1-trim69 + MO2-jun standard conditions Prim-5 brain ISH
sox2 WT control Prim-5 brain ISH
WT + MO1-trim69 + MO2-jun standard conditions Prim-5 brain ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-trim69 + MO2-jun standard conditions Prim-5 brain ascl1a expression amount, ameliorated
WT + MO1-trim69 + MO2-jun standard conditions Prim-5 brain pax3a expression amount, ameliorated
WT + MO1-trim69 + MO2-jun standard conditions Prim-5 brain pax6a expression amount, ameliorated
WT + MO1-trim69 + MO2-jun standard conditions Prim-5 brain nanog expression amount, ameliorated
WT + MO1-trim69 + MO2-jun standard conditions Prim-5 brain sox2 expression amount, ameliorated
WT + MO1-trim69 + MO2-jun standard conditions Prim-5 brain pax7a expression amount, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.