ZFIN ID: ZDB-FIG-131227-1
Seredick et al., 2012 - Zebrafish Mnx proteins specify one motoneuron subtype and suppress acquisition of interneuron characteristics. Neural Development   7(1):35 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 26+ somites

Fig. 5 Mnx proteins prevent MiPs from acquiring V2a-like molecular characteristics. (A-D) Single spinal hemisegments of control and mnx MO-injected embryos. MiPs (closed arrowheads) and CaPs (open arrowheads) are indicated in merged panels (right column). (A) In control embryos, MiPs and CaPs express chat but never vglut (0/20 vglut+ MiPs or CaPs in five embryos). (B) In mnx MO-injected embryos, MiPs co-express both chat and vglut (22/41 vglut+ MiPs in eight embryos), while CaPs express chat but not vglut (0/41 vglut+ CaPs in eight embryos. (C) In control embryos, MiPs express islet1 but not vsx2 (0/50 vsx2+ MiPs in 10 embryos), while CaPs express neither islet1 nor vsx2 (0/50 vsx2+ CaPs in 10 embryos). (D) In mnx MO-injected embryos, MiP express vsx2 but not islet1 (26/60 vsx2+ MiPs in 13 embryos) while CaPs express neither vsx2 nor islet1 (0/60 vsx2+ CaPs in 13 embryos). Scale bar: 10 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
chata js12Tg standard conditions 26+ somites CaP motoneuron IHC
26+ somites MiP motor neuron IHC
js12Tg + MO1-mnx2a + MO1-mnx2b + MO5-mnx1 standard conditions 26+ somites CaP motoneuron IHC
26+ somites MiP motor neuron IHC
GFP js12Tg standard conditions 26+ somites primary motor neuron IHC
js12Tg + MO1-mnx2a + MO1-mnx2b + MO5-mnx1 standard conditions 26+ somites primary motor neuron IHC
isl1 js12Tg standard conditions 26+ somites Not Detected CaP motoneuron ISH
26+ somites MiP motor neuron ISH
js12Tg + MO1-mnx2a + MO1-mnx2b + MO5-mnx1 standard conditions 26+ somites Not Detected CaP motoneuron ISH
26+ somites Not Detected MiP motor neuron ISH
slc17a6a js12Tg standard conditions 26+ somites Not Detected CaP motoneuron ISH
26+ somites Not Detected MiP motor neuron ISH
js12Tg + MO1-mnx2a + MO1-mnx2b + MO5-mnx1 standard conditions 26+ somites Not Detected CaP motoneuron ISH
26+ somites MiP motor neuron ISH
slc17a6b js12Tg standard conditions 26+ somites Not Detected CaP motoneuron ISH
26+ somites Not Detected MiP motor neuron ISH
js12Tg + MO1-mnx2a + MO1-mnx2b + MO5-mnx1 standard conditions 26+ somites Not Detected CaP motoneuron ISH
26+ somites MiP motor neuron ISH
slc17a7a js12Tg standard conditions 26+ somites Not Detected CaP motoneuron ISH
26+ somites Not Detected MiP motor neuron ISH
js12Tg + MO1-mnx2a + MO1-mnx2b + MO5-mnx1 standard conditions 26+ somites Not Detected CaP motoneuron ISH
26+ somites MiP motor neuron ISH
vsx2 js12Tg standard conditions 26+ somites Not Detected CaP motoneuron ISH
26+ somites Not Detected MiP motor neuron ISH
js12Tg + MO1-mnx2a + MO1-mnx2b + MO5-mnx1 standard conditions 26+ somites Not Detected CaP motoneuron ISH
26+ somites MiP motor neuron ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Neural Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neural Dev.