ZFIN ID: ZDB-FIG-130401-19
Seredick et al., 2012 - Zebrafish Mnx proteins specify one motoneuron subtype and suppress acquisition of interneuron characteristics. Neural Development   7(1):35 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 14-19 somites to 26+ somites

Fig. 6 Mnx proteins maintain the late phase of Islet1 expression in MiPs independently of Nkx6. (A-D) Single spinal hemisegments of control and mnx MO-injected embryos. MiPs (closed arrowheads) and CaPs (open arrowheads) are indicated in each of the single channel panels; right column shows merged channels. (A) At 17 hpf, MiPs in control embryos strongly express Islet and Nkx6 (17/30 Nkx6+ MiPs in seven embryos). CaPs strongly express Islet but weakly express Nkx6. (B) MiPs in mnx MO-injected embryos continue to strongly express Islet and Nkx6 (13/22 Nkx6+ MiPs in five embryos). (C) At 21 hpf in Tg(nrp1a:GFP) control embryos, both MiPs and CaPs express Islet (32/32 Islet+ CaPs, and 31/32 Islet+ in five embryos. (D) In mnx MO-injected Tg(nrp1a:GFP) embryos, CaPs (60/60 Islet+ CaPs in 10 embryos) but not MiPs (19/60 Islet+ MiPs in 10 embryos), strongly express Islet. Scale bar: 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
GFP js12Tg standard conditions 26+ somites primary motor neuron IHC
js12Tg + MO1-mnx2a + MO1-mnx2b + MO5-mnx1 standard conditions 26+ somites primary motor neuron IHC
isl1 WT standard conditions 14-19 somites CaP motoneuron ISH
14-19 somites MiP motor neuron ISH
WT + MO1-mnx2a + MO1-mnx2b + MO5-mnx1 standard conditions 14-19 somites CaP motoneuron ISH
14-19 somites MiP motor neuron ISH
js12Tg standard conditions 26+ somites CaP motoneuron ISH
26+ somites MiP motor neuron ISH
js12Tg + MO1-mnx2a + MO1-mnx2b + MO5-mnx1 standard conditions 26+ somites CaP motoneuron ISH
26+ somites Not Detected MiP motor neuron ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-nkx6 IHC js12Tg standard conditions 14-19 somites CaP motoneuron
IHC 14-19 somites MiP motor neuron
IHC js12Tg + MO1-mnx2a + MO1-mnx2b + MO5-mnx1 standard conditions 14-19 somites CaP motoneuron
IHC 14-19 somites MiP motor neuron
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Neural Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neural Dev.