ZFIN ID: ZDB-FIG-130401-8
Seredick et al., 2012 - Zebrafish Mnx proteins specify one motoneuron subtype and suppress acquisition of interneuron characteristics. Neural Development   7(1):35 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: 10-13 somites to Prim-5

Fig. 1 Zebrafish mnx family genes are dynamically expressed in PMNs and VeLD interneurons. Lateral views, anterior to the left; all figures are in this orientation unless otherwise noted. MiPs and RoPs are outlined green in panels with islet1 labeling, CaPs and VaPs are outlined blue in panels with islet2a labeling. Segment boundaries are demarcated with diagonal lines. (A-L) RNA expression; two spinal hemisegments are shown in each panel. At 14 hpf, mnx1 is co-expressed with both islet1 (A) and islet2a (B), mnx2a is only co-expressed with islet2a (E, F), and mnx2b is co-expressed with both islet1 (I) and islet2a (J). The VeLD interneuron can be seen expressing both mnx1 (arrowheads A, B) and mnx2b (arrowheads I, J). At 24 hpf, mnx1 maintains co-expression with both islet1 (C) and islet2a (D), mnx2a is co-expressed with both islet1 (G) and islet2a (H), and mnx2b is co-expressed only with islet1 (K, L). VeLD still expresses mnx1 (arrowhead C). (M-N) Protein expression. Mnx2b protein (red) co-localizes with the MiP soma (GFP; green) (M). Mnx1 protein (red) co-localizes with the VeLD soma (GFP; green) (N). (O) Schematic of mnx expression. Scale bar: 10 μm, A-L; 10 μmm M; 20 μm N.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
isl1 WT standard conditions 10-13 somites primary motor neuron ISH
Prim-5 MiP motor neuron ISH
Prim-5 RoP motor neuron ISH
isl2a WT standard conditions 10-13 somites primary motor neuron ISH
Prim-5 CaP motoneuron ISH
Prim-5 VaP motor neuron ISH
mnx1 WT standard conditions 10-13 somites to Prim-5 CaP motoneuron ISH
10-13 somites to Prim-5 MiP motor neuron ISH
10-13 somites to Prim-5 primary motor neuron ISH
10-13 somites to Prim-5 RoP motor neuron ISH
10-13 somites to Prim-5 VaP motor neuron ISH
mnx2a WT standard conditions 10-13 somites to 14-19 somites CaP motoneuron ISH
10-13 somites to 14-19 somites VaP motor neuron ISH
20-25 somites to Prim-5 CaP motoneuron ISH
20-25 somites to Prim-5 MiP motor neuron ISH
20-25 somites to Prim-5 RoP motor neuron ISH
20-25 somites to Prim-5 VaP motor neuron ISH
mnx2b WT standard conditions 10-13 somites to 14-19 somites CaP motoneuron ISH
10-13 somites to 14-19 somites MiP motor neuron ISH
10-13 somites to 14-19 somites RoP motor neuron ISH
10-13 somites to 14-19 somites VaP motor neuron ISH
20-25 somites to Prim-5 MiP motor neuron ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-mnx2b IHC b1222Tg standard conditions Prim-5 MiP motor neuron
Ab2-mnx1 IHC b1222Tg standard conditions Prim-5 VeLD
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Neural Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neural Dev.