ZFIN ID: ZDB-FIG-130401-15
Seredick et al., 2012 - Zebrafish Mnx proteins specify one motoneuron subtype and suppress acquisition of interneuron characteristics. Neural Development   7(1):35 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-15
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 14-19 somites to Prim-15

Fig. 3 Mnx proteins promote MiP subtype identity and prevent interneuron-like and CaP-like processes. (A-D) Control and mnx MO-injected embryos. (A) In controls islet1 and islet2a expression is mutually exclusive at 18 hpf. (B) In embryos injected with mnx1, mnx2a, and mnx2b MOs, islet1 is co-expressed with islet2a in CaP and VaP. (C) Control embryos have normal, ventrally-projecting CaP axons (arrows) and dorsally-projecting MiP axons (arrowheads). (D) CaP axons (arrows) are normal in mnx MO-injected embryos whereas MiP axons (closed arrowheads) are absent and there are ectopic interneuron-like axons (open arrowheads). (E-J′′) Rhodamine-dextran-labeled MiPs in 28 to 32 hpf Tg(nrp1a:GFP) embryos injected with mnx1, mnx2a, and mnx2b MOs. Panels show rhodamine-dextran labeling (no superscript), nrp1a:GFP (2) and a merged image of the two channels (′′). Labeled MiPs (yellow arrowheads) become hybrids with six morphologies. (E-E′′) V2a hybrids express nrp1a:GFP and have a descending V2a-like axon. (F-F′′) MiP-V2a hybrids have a descending V2a-like axon as well as a normal-appearing MiP ventral axon; the red blob to the right is a cell that was killed during labeling. (G-G′′) MiP-V2a-CaP hybrids have a descending V2a-like axon as well as a ventrally-projecting CaP-like axon. (H-H′′) MiP-CaP hybrids have both a normal MiP axon and a CaP-like axon. (I-I′′) Truncated MiP-CaP hybrids have a truncated MiP dorsal axon as well as a ventrally-projecting CaP-like axon. (J-J′′) CaP hybrids have a CaP-like axon that extends next to a normal CaP axon. Scale bar: 20 μm, A-D; 40 μm, E-H′′.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP js12Tg standard conditions 14-19 somites primary motor neuron IHC
14-19 somites primary motor neuron neuron projection IHC
Prim-5 primary motor neuron IHC
Prim-5 primary motor neuron neuron projection IHC
Prim-15 primary motor neuron IHC
Prim-15 primary motor neuron neuron projection IHC
js12Tg + MO1-mnx2a + MO1-mnx2b + MO5-mnx1 standard conditions 14-19 somites primary motor neuron IHC
14-19 somites primary motor neuron neuron projection IHC
Prim-5 primary motor neuron IHC
Prim-5 primary motor neuron neuron projection IHC
Prim-15 primary motor neuron IHC
Prim-15 primary motor neuron neuron projection IHC
isl1 WT standard conditions 14-19 somites MiP motor neuron ISH
14-19 somites RoP motor neuron ISH
WT + MO1-mnx2a + MO1-mnx2b + MO5-mnx1 standard conditions 14-19 somites CaP motoneuron ISH
isl2a WT standard conditions 14-19 somites CaP motoneuron ISH
14-19 somites VaP motor neuron ISH
WT + MO1-mnx2a + MO1-mnx2b + MO5-mnx1 standard conditions 14-19 somites CaP motoneuron ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
js12Tg + MO1-mnx2a + MO1-mnx2b + MO5-mnx1 standard conditions 14-19 somites MiP motor neuron lacks parts or has fewer parts of type MiP motor neuron axon, abnormal
14-19 somites MiP motor neuron axon mislocalised, abnormal
14-19 somites MiP motor neuron axon physical object quality, abnormal
14-19 somites MiP motor neuron axon truncated, abnormal
Prim-5 MiP motor neuron lacks parts or has fewer parts of type MiP motor neuron axon, abnormal
Prim-5 MiP motor neuron axon mislocalised, abnormal
Prim-5 MiP motor neuron axon physical object quality, abnormal
Prim-5 MiP motor neuron axon truncated, abnormal
Prim-15 MiP motor neuron lacks parts or has fewer parts of type MiP motor neuron axon, abnormal
Prim-15 MiP motor neuron axon mislocalised, abnormal
Prim-15 MiP motor neuron axon physical object quality, abnormal
Prim-15 MiP motor neuron axon truncated, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Neural Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neural Dev.