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ZFIN ID: ZDB-FIG-121101-23
Cox et al., 2012 - An essential role of variant histone h3.3 for ectomesenchyme potential of the cranial neural crest. PLoS Genetics   8(9):e1002938 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 1-4 somites to 14-19 somites

Fig. 6 H3.3 functions at the NPB–CNC transition.

a–f, Expression of msxb, pax3a, zic2a, and tfap2a at 10 hpf and msxb and pax3a at 11 hpf is indistinguishable between wild types and both homozygous and heterozygous h3f3adb1092 mutants (mut) (n≥10 for each). g–k, At 11 hpf, both homozygous and heterozygous h3f3adb1092 mutants have severe reductions in the expression of snai2 (4/4 mut; 0/4 wt), sox10 (10/12 mut; 0/6 wild-type), foxd3 (9/9 mut; 0/5 wt), tfap2a (7/7 mut; 0/4 wt), and sox9b (8/8 mut; 0/3 wt). l, sox10 expression is also lost in embryos injected with D123N (10/12) but not wild-type (0/16) h3f3a mRNA. m, In both homozygous and heterozygous h3f3adb1092 embryos, sox10 expression partially recovers by 16.5 hpf yet is specifically reduced in presumptive CNC ectomesenchyme domains (arrows) (6/6 mut; 0/4 wt). An increase in sox10-positive cells is evident in the mutant dorsal neural tube (insert) between the sox10-positive otic placodes (arrowheads) which are unaffected in mutants. n, At 16.5 hpf, dlx2a expression in three streams of migrating ectomesenchyme is reduced in both homozygous and heterozygous h3f3adb1092 mutants (6/7 mut; 0/5 wt). o, The 16.5 hpf ectomesenchyme expression (arrows) of twist1a is reduced in both homozygous and heterozygous h3f3adb1092 mutants yet paraxial mesoderm expression is unaffected (white arrowheads) (8/8 mut; 0/5 wt). In all panels, homozygous h3f3adb1092 examples are shown. All images are dorsal views with anterior to the left. Scale bars: 250 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dlx2a WT standard conditions 14-19 somites forebrain neural tube ISH
h3f3adb1092/+ standard conditions 14-19 somites forebrain neural tube ISH
foxd3 WT standard conditions 1-4 somites cranial neural crest ISH
h3f3adb1092/+ standard conditions 1-4 somites Not Detected cranial neural crest ISH
msx1b WT standard conditions Bud neural plate ISH
1-4 somites neural plate ISH
h3f3adb1092/+ standard conditions 1-4 somites neural plate ISH
Bud neural plate ISH
pax3a WT standard conditions Bud neural plate ISH
1-4 somites neural plate ISH
h3f3adb1092/+ standard conditions 1-4 somites neural plate ISH
Bud neural plate ISH
snai1b WT standard conditions 1-4 somites cranial neural crest ISH
h3f3adb1092/+ standard conditions 1-4 somites Not Detected cranial neural crest ISH
sox9b WT standard conditions 1-4 somites cranial neural crest ISH
h3f3adb1092/+ standard conditions 1-4 somites Not Detected cranial neural crest ISH
sox10 WT standard conditions 1-4 somites cranial neural crest ISH
14-19 somites otic placode ISH
h3f3adb1092/+ standard conditions 1-4 somites Not Detected cranial neural crest ISH
14-19 somites otic placode ISH
tfap2a WT standard conditions Bud neural plate ISH
1-4 somites neural plate ISH
h3f3adb1092/+ standard conditions 1-4 somites Not Detected neural plate ISH
Bud neural plate ISH
twist1a WT standard conditions 14-19 somites mesenchyme ISH
14-19 somites paraxial mesoderm ISH
h3f3adb1092/+ standard conditions 14-19 somites mesenchyme ISH
14-19 somites paraxial mesoderm ISH
zic2a WT standard conditions Bud neural plate ISH
h3f3adb1092/+ standard conditions Bud neural plate ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.