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ZIRC
ZFIN ID: ZDB-FIG-121101-24
Cox et al., 2012 - An essential role of variant histone h3.3 for ectomesenchyme potential of the cranial neural crest. PLoS Genetics   8(9):e1002938 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: 5-9 somites

Fig. 7 Trunk NC is largely unaffected in h3f3adb1092 mutants.

a–c, crestin expression at 11.7 hpf shows similar amounts of trunk NC in wild-type and both homozygous and heterozygous h3f3adb1092 embryos (n = 5 for each genotype). d–i, Trunk views of sox9b expression at 11.7 hpf show that trunk NC specification is largely normal in both homozygous and heterozygous h3f3adb1092 mutants (n = 10 for each genotype). Cranial views of the same embryos show reduced amounts of sox9b-expressing CNC. Arrows show the sox9b-positive otic placodes that are unaffected in mutants. Scale bars = 250 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
crestin WT standard conditions 5-9 somites trunk neural crest cell ISH
h3f3adb1092/+ standard conditions 5-9 somites trunk neural crest cell ISH
h3f3adb1092/db1092 standard conditions 5-9 somites trunk neural crest cell ISH
sox9b WT standard conditions 5-9 somites cranial neural crest ISH
5-9 somites otic placode ISH
5-9 somites trunk neural crest cell ISH
h3f3adb1092/+ standard conditions 5-9 somites cranial neural crest ISH
5-9 somites otic placode ISH
5-9 somites trunk neural crest cell ISH
h3f3adb1092/db1092 standard conditions 5-9 somites cranial neural crest ISH
5-9 somites otic placode ISH
5-9 somites trunk neural crest cell ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.