ZFIN Figure: Cox et al., 2012, Fig. 7

ZFIN ID: ZDB-FIG-121101-24

ZFIN ID: ZDB-FIG-121101-24

Cox et al., 2012 - An essential role of variant histone h3.3 for ectomesenchyme potential of the cranial neural crest. PLoS Genetics 8(9):e1002938 Full text @ PLoS Genet.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	crestin sox9b
Fish:	WT h3f3a^{db1092/db1092} h3f3a^db1092/+
Anatomical Terms:	cranial neural crest otic placode trunk neural crest cell
Stage:	5-9 somites

Fig. 7 Trunk NC is largely unaffected in h3f3a^db1092 mutants.

a–c, crestin expression at 11.7 hpf shows similar amounts of trunk NC in wild-type and both homozygous and heterozygous h3f3a^db1092 embryos (n = 5 for each genotype). d–i, Trunk views of sox9b expression at 11.7 hpf show that trunk NC specification is largely normal in both homozygous and heterozygous h3f3a^db1092 mutants (n = 10 for each genotype). Cranial views of the same embryos show reduced amounts of sox9b-expressing CNC. Arrows show the sox9b-positive otic placodes that are unaffected in mutants. Scale bars = 250 μm.

Gene Expression Details

Gene	Fish	Conditions	Stage	Anatomy	Assay
crestin	WT	standard conditions	5-9 somites	trunk neural crest cell	ISH
	h3f3a^db1092/+	standard conditions	5-9 somites	trunk neural crest cell	ISH
	h3f3a^{db1092/db1092}	standard conditions	5-9 somites	trunk neural crest cell	ISH
sox9b	WT	standard conditions	5-9 somites	cranial neural crest	ISH
			5-9 somites	otic placode	ISH
			5-9 somites	trunk neural crest cell	ISH
	h3f3a^db1092/+	standard conditions	5-9 somites	cranial neural crest	ISH
			5-9 somites	otic placode	ISH
			5-9 somites	trunk neural crest cell	ISH
	h3f3a^{db1092/db1092}	standard conditions	5-9 somites	cranial neural crest	ISH
			5-9 somites	otic placode	ISH
			5-9 somites	trunk neural crest cell	ISH

Antibody Labeling Details No data available

Phenotype Details No data available

Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.