ZFIN Figure: Cox et al., 2012, Fig. 9

ZFIN ID: ZDB-FIG-121101-27

ZFIN ID: ZDB-FIG-121101-27

Cox et al., 2012 - An essential role of variant histone h3.3 for ectomesenchyme potential of the cranial neural crest. PLoS Genetics 8(9):e1002938 Full text @ PLoS Genet.

ADDITIONAL FIGURES

PHENOTYPE:

Fish:	h3f3a^{db1092/db1092} h3f3a^db1092/+
Observed In:	neural tube dorsal region
Stage:	14-19 somites

Fig. 9 Cell death in h3f3a^db1092 embryos.

a–c, Lysotracker Red staining marks similar amounts of dying cells in wild-type (n = 2), h3f3a^db1092/+ heterozygous (n = 5), and h3f3a^{db1092/db1092} homozygous (n = 3) embryos at 12.5 hpf. The bright staining in the bottom of each panel is the yolk. d–f, At 16 hpf, increased Lysotracker Red staining (arrows) was evident in the dorsal neural tube of h3f3a^db1092/+ heterozygotes (2/2) and h3f3a^{db1092/db1092} homozygotes (3/3) but not wild types (0/4). These dying cells were located in a similar position to where CNC forms in wild-type embryos. Scale bar = 50 μm.

Gene Expression Details No data available

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
h3f3a^db1092/+	standard conditions	14-19 somites	neural tube dorsal region necrotic, abnormal
h3f3a^{db1092/db1092}	standard conditions	14-19 somites	neural tube dorsal region necrotic, abnormal

Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.