ZFIN ID: ZDB-FIG-060808-14
Peng et al., 2006 - Lhx5 promotes forebrain development and activates transcription of secreted Wnt antagonists. Development (Cambridge, England)   133(16):3191-3200 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Shield to 10-13 somites

Fig. 1 Lhx5 promotes forebrain development. In this and subsequent figures, the probes used for whole-mount in situ hybridization are listed in the upper right corner of each panel. Genotypes or experimental manipulations are indicated in the lower left corners. Developmental stages are indicated in the lower right corners. Unless otherwise noted, gastrula stage embryos are orientated in animal pole view, rostral to the top; post-gastrulation stage embryos in lateral view, rostral to the top and dorsal to the right. ctl, control embryos. (A-D) lhx5 is expressed in rostral regions during embryonic development. (A,B) Dorsal to the right, lateral (A) and animal pole (B) views. (D) A gap can be seen between the lhx5 and pax2a expression domains. (E-N) lhx5 gain of function causes expansion of the forebrain. (E,F) Forebrain boundaries are marked by broken lines. (G,H) Dorsal view, bud stage, pax6a expression. (I-N) Dorsal view, rostral to the top. Embryos were dissected and flat mounted in glycerol after in situ hybridization. Partial overlays of panels were made with PhotoShop (Adobe) and brightness in the overlapped regions was adjusted so that the backgrounds match. (O-V) Inhibition of Lhx5 function compromises forebrain development. (U,V) Red arrowheads mark the forebrain-midbrain boundary. (W-Z) lhx5 morpholino knockdown alters pax6a and pax2a expression. Embryos were dissected and flat mounted in glycerol after in situ hybridization. Dorsal view, rostral to the top. Scale bar in Z: 250 μm for A-H,Q-V; 200 μm for I-K; 167 μm for L-N; 150 μm for O,P; 100 μm for W-Z.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
egr2b WT standard conditions Bud midbrain hindbrain boundary neural plate ISH
emx3 WT standard conditions Bud neural keel ISH
lhx5 WT standard conditions Shield presumptive ectoderm ISH
Bud neural keel ISH
pax2a WT standard conditions Bud midbrain hindbrain boundary neural plate ISH
10-13 somites optic vesicle ISH
WT + MO1-lhx5 standard conditions 10-13 somites optic vesicle ISH
pax6a WT standard conditions Bud neural plate ISH
10-13 somites optic vesicle ISH
WT + MO1-lhx5 standard conditions 10-13 somites optic vesicle ISH
six3b WT standard conditions Bud neural keel ISH
wnt8b WT standard conditions 10-13 somites forebrain midbrain boundary neural rod ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development