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ZIRC
ZFIN ID: ZDB-FIG-060808-225
Peng et al., 2006 - Lhx5 promotes forebrain development and activates transcription of secreted Wnt antagonists. Development (Cambridge, England)   133(16):3191-3200 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Bud

Fig. 4 Sfrps antagonize Wnt signaling and partially restore forebrain marker expression in mbl mutant embryos. (A) Sfrp1a and Sfrp5 rescue eye development in wnt8a mRNA-injected embryos. Injected embryos were scored after 24 hours post-fertilization. (B-D) sfrp1a or sfrp5 gain of function expands the six3b expression domain. (E-G) sfrp1a or sfrp5 gain of function expands the emx3 expression domain. Embryos were dissected and flat mounted in glycerol after in situ hybridization. Animal pole views, rostral to the top. (H-M) Overexpression of Sfrps partially restores emx3 (J) and six3b (M) expression in mbl embryos. Dosage injected was 150 pg of sfrp1a mRNA and 100 pg of sfrp5 mRNA per embryo. Genotypes of mutant and partially rescued embryos were verified by RFLP. WT, wild-type embryos. Scale bar in M: 250 μM for B-M.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
emx3 WT standard conditions Bud neural keel ISH
axin1tm213/tm213 standard conditions Bud Not Detected neural keel ISH
six3b WT standard conditions Bud neural keel ISH
axin1tm213/tm213 standard conditions Bud Not Detected neural keel ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development