ZFIN ID: ZDB-FIG-060808-226
Peng et al., 2006 - Lhx5 promotes forebrain development and activates transcription of secreted Wnt antagonists. Development (Cambridge, England)   133(16):3191-3200 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Shield to Prim-5

Fig. 5 Lhx5 regulates Sfrp1a and Sfrp5 expression. (A-I) Lhx5 regulates Sfrp1a expression. (A-C) Animal pole views, dorsal to the right. (D-F) Dorsal views, bud stage, sfrp1a expression. (G-I) Flat mounts in glycerol; dorsal views, rostral to the top. 2 ng (H) or 4 ng (I) of lhx5 morpholino was injected. (J-M) Lhx5 cell autonomously regulates Sfrp1a expression. Transplanted cells are labeled in red. sfrp1a transcripts are highlighted by white arrowheads and are apparent as blue dots in the narrow cytoplasm around the nuclei of transplanted cells in controls (J, ctl) and lhx5 mRNA-injected cells transplanted into lhx5 morpholino injected hosts (M). sfrp1a transcripts are not detected in lhx5-en injected cells transplanted into control hosts (K). In transplanted lhx5 morpholino-injected cells, sfrp1a expression is reduced (L). (J-L) 90% epiboly stage; (M) one-somite stage. (N) Mapping of sfrp1a upstream enhancers by transient GFP reporter expression in the forebrain. Fragments are numbered using the first nucleotide of the Sfrp1a start codon as the reference. GFP expression in the forebrain was scored at the 3 somite stage and the Prim-5 stage. The number of + signs indicate relative GFP expression levels. (O) Lhx5 binds to the sfrp1a promoter. Input represents 0.2% (2 μl, 2x) or 0.1% (1 μl, 1x) of starting material for each immunoprecipitation reaction. 2 μl (2x) or 1 μl (1x) of purified DNA product from each sample was used in the PCR. ctl., uninjected embryos treated in parallel; inj., lhx5-GFP mRNA-injected embryos. Mock immunoprecipitation with no antibody fails to amplify from either uninjected or injected groups (data not shown). (P-U) Lhx5 regulates Sfrp5 expression. Flat mounts in glycerol, dorsal views, rostral to the top. (R,T,U) 2 ng (R,T) or 4 ng (U) of lhx5 morpholinos was injected. Scale bar in U: 250 μm for A-F; 100 μm for G-I; 8 μm for J-M; 100 μm for P-U.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
sfrp1a WT standard conditions Shield margin ISH
Shield presumptive ectoderm ISH
Bud mesoderm ISH
Bud neural keel ISH
Bud neural plate ISH
Prim-5 forebrain ISH
WT + MO1-lhx5 standard conditions Bud mesoderm ISH
Bud Not Detected neural keel ISH
Bud neural plate ISH
WT + MO1-lhx5 standard conditions Prim-5 forebrain ISH
WT + MO2-lhx5 standard conditions Bud mesoderm ISH
Bud Not Detected neural keel ISH
Bud neural plate ISH
sfrp5 WT standard conditions 5-9 somites forebrain neural rod ISH
Prim-5 forebrain ISH
WT + MO1-lhx5 standard conditions 5-9 somites forebrain neural rod ISH
Prim-5 forebrain ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development