ZFIN ID: ZDB-FIG-060808-227
Peng et al., 2006 - Lhx5 promotes forebrain development and activates transcription of secreted Wnt antagonists. Development (Cambridge, England)   133(16):3191-3200 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Shield to 14-19 somites

Fig. 6 Sfrp compensates for inhibition of Lhx5 function.(A) Sfrp1a and Sfrp5 rescue eye development in lhx5-en mRNA-injected embryos. The percentages of embryos malformed, as a result of dorsalization or injection (yellow), eyeless (blue) or two eyed (green) were measured. (B-E) Ectopic Wnt signaling results in ectopic axin2 expression. (B,C) Animal pole views; (D,E) lateral views, rostral to the left, dorsal to the top. Genotypes were determined by RFLP. (F-I) Ectopic axin2 expression in lhx5 morpholino-injected embryos. Flat mounts in glycerol. Brain boundaries are marked by broken lines. Green arrows indicate the borders of axin2 labeling. (F,G) Dorsal views, rostral to the top; (H,I) ateral views, rostral to the left, dorsal to the top. Eyes were removed to expose the brain. (J-M) sfrp1a or sfrp5 gain of function reduces ectopic axin2 expression in lhx5-en-injected embryos. Animal pole views, dorsal to the left. Scale bar in M: 250 μm for B,C; 125 μm for D,E; 100 μm for F-I; 250 μm for J-M.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
axin2 WT standard conditions Shield margin ISH
1-4 somites forebrain neural keel ISH
14-19 somites brain ISH
WT + MO1-lhx5 standard conditions 14-19 somites brain ISH
14-19 somites forebrain ISH
axin1tm213/tm213 standard conditions 1-4 somites forebrain neural keel ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development