ZFIN ID: ZDB-FIG-060808-228
Peng et al., 2006 - Lhx5 promotes forebrain development and activates transcription of secreted Wnt antagonists. Development (Cambridge, England)   133(16):3191-3200 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Bud to 14-19 somites

Fig. S1 Efficacy and sepcificity of lhx5 morpholinos. (A-D) lhx5 morpholino knockdown alters pax6a and pax2a expression patterns. Embryos were dissected and flat mounted in glycerol after in situ hybridization. Dorsal views, rostral to the top. (E-G) lhx5 translation-blocking morpholino reduces lhx5-GFP mRNA translation. GFP fluorescence in the cell nucleus is reduced when 2 ng (F) or 4 ng (G) of the translational morpholino is injected into zebrafish embryos. (H) lhx5-splicing morpholino reduces wild-type lhx5 transcripts. Arrow indicates wild-type lhx5 or odc1 transcripts. Band above the wild-type lhx5 transcript is caused by splicing with a cryptic GT located in lhx5 intron 3 downstream from the canonical GT site at the exon 3/intron 3 junction; it results in a frame shift. Amplifications of odc1 transcripts serve as controls.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
lhx5 WT standard conditions Bud whole organism RTPCR
WT + MO2-lhx5 standard conditions Bud whole organism RTPCR
odc1 WT standard conditions Bud whole organism RTPCR
WT + MO2-lhx5 standard conditions Bud whole organism RTPCR
pax2a WT standard conditions 10-13 somites optic vesicle ISH
WT + MO2-lhx5 standard conditions 10-13 somites optic vesicle ISH
pax6a WT standard conditions 14-19 somites optic vesicle ISH
WT + MO2-lhx5 standard conditions 14-19 somites optic vesicle ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development