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Disruption of sema4ab impairs recovery of swimming behavior.
(A) A test of swimming behavior at 5 dpf in unlesioned larvae shows no change in swimming distance in sema4ab germline mutants, compared to wildtype controls (wild type: 128.8 mm/min ± 6.657; sema4ab −/−: 116.2 mm/min ± 6.504; Unpaired t test: p = 0.1798). (B) A test of swimming behavior at 48 hpl in 5 dpf larvae shows a reduction in swimming distance in sema4ab germline mutants (wild type: 76.98 mm/min ± 8.846; sema4ab −/−: 42.15 mm/min ± 5.508; Mann–Whitney U test: p = 0.0005). (C) In sema4ab mutants the axonal fiber bundle crossing the injury site shows reduced thickness, compared to controls at 48 hpl (wild type: 20.20 µm ± 1.5; sema4ab −/−: 15.8 µm ± 1.7; one-tailed t test: p = 0.0253). Error bars show SEM. Scale bars: 50 µm. Data files for graphs available in S2 Data.
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