FIGURE

Fig S10

ID

ZDB-FIG-260821-33

Publication

Docampo-Seara et al., 2026 - The microglia-derived protein sema4ab attenuates regenerative neurogenesis after spinal cord injury in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig S10

Microglia depletion increases tgfb3 levels while cytokine/cytokine receptor loss of function drives no changes. (A–C) A Microglia depletion line shows an increase in tgfb3 levels at 24 hpl by qRT-PCR (One-sample t test: p = 0.0281). (D, E) Impairing cytokine signaling by YVAD (impairs il1b cleavage) and tnfrsf1a loss of function, results in no meaningful changes in tgfb3 levels at 24 hpl by qRT-PCR (One-sample t test; YVAD: p = 0.2303; gTnfrsf1a: p = 0.6461). (F) Feature plots showing main pro-inflammatory cytokine receptor expression pattern. Note a strong expression in fibroblasts (circle). Each dot for qRT-PCR represents a pool of 50 larvae. β-actin was used as housekeeping gene. Raw data for qRT-PCR can be found in S5 and S6 Data. Scale bars: 50 µm. Data files for graphs available in S3 Data.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Biol.