FIGURE

Fig 3

ID

ZDB-FIG-260629-133

Publication

Docampo-Seara et al., 2026 - The microglia-derived protein sema4ab attenuates regenerative neurogenesis after spinal cord injury in zebrafish

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Fig 3

Sema4ab is expressed mainly in microglia in the spinal injury site.
(A) Experimental timeline and schematic of a zebrafish larva indicating the lateral view of the spinal injury site shown throughout the article (unless specified differently) are shown. (B) A time-course analysis of HCR-FISH indicates increased expression of sema4ab during spinal cord repair. (C) Triple-multiplexed HCR-FISH at 24 hpl shows co-localization of sema4ab, mpeg1:mCherry, and apoeb at 24 hpl (blue arrowheads). (D) High magnifications of apoeb/mpeg1:mCherry/sema4ab labeling to differentiate blood-derived macrophages (BDMs; apoeb−) from microglia (apoeb+) for cell counting. (E) Quantifications at 24 hpl show that most sema4ab-expressing cells are microglia (apoeb+/mpeg1:mCherry+/sema4ab+). Error bars show SEM. Asterisks indicate the injury site. Scale bars: 100 µm (B, C), 15 µm (D). Data files for graphs available in S2 Data.

Expression Data

Gene:	sema4ab
Fish:	AB
Condition:	transection: spinal cord
Anatomical Term:	spinal cord microglial cell
Stage Range:	Protruding-mouth to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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