ZFIN ID: ZDB-FIG-200530-24
Kantarci et al., 2020 - The Warburg Effect and lactate signaling augment Fgf-MAPK to promote sensory-neural development in the otic vesicle. eLIFE   9 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: 14-19 somites to Long-pec

Figure 2

Sensory development and early Fgf signaling are impaired in sagd1 mutants.

(A–D”, F–T’) Dorsolateral views of whole mount specimens (anterior to the left) showing expression of the indicated genes in the otic vesicle (outlined) at the indicated times in wild-type embryos and sagd1 mutants. (E) Mean and standard deviation of hair cells in the anterior/utricular and posterior/auditory maculae at 36 and 48 hpf in wild-type embryos (black) and sagd1 mutants (red). Sample sizes are indicated.


Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
atoh1a AB control Prim-5 anterior macula ISH
Prim-5 posterior macula ISH
Prim-15 anterior macula ISH
Prim-15 posterior macula ISH
pgk1x40/x40 control Prim-5 anterior macula ISH
Prim-5 posterior macula ISH
Prim-15 anterior macula ISH
Prim-15 posterior macula ISH
dlx3b AB control Prim-15 otic vesicle dorsal region ISH
pgk1x40/x40 control Prim-15 otic vesicle dorsal region ISH
etv5b AB control 14-19 somites otic vesicle ISH
20-25 somites otic vesicle ISH
Prim-5 otic vesicle ISH
pgk1x40/x40 control 14-19 somites otic vesicle ISH
20-25 somites otic vesicle ISH
Prim-5 otic vesicle ISH
fgf3 AB control Prim-15 otic vesicle ISH
pgk1x40/x40 control Prim-15 otic vesicle ISH
fgf8a AB control Prim-15 otic vesicle ISH
pgk1x40/x40 control Prim-15 otic vesicle ISH
GFP pgk1x40/x40; s356tTg control Prim-25 otic vesicle hair cell IFL
Long-pec otic vesicle hair cell IFL
s356tTg control Prim-25 otic vesicle hair cell IFL
Long-pec otic vesicle hair cell IFL
otx1 AB control Prim-15 otic vesicle ventro-lateral region ISH
pgk1x40/x40 control Prim-15 otic vesicle ventro-lateral region ISH
pax5 AB control 14-19 somites otic vesicle ISH
20-25 somites otic vesicle ISH
Prim-5 otic vesicle ISH
Prim-15 otic vesicle antero-medial region ISH
pgk1x40/x40 control 14-19 somites otic vesicle ISH
20-25 somites otic vesicle ISH
Prim-5 otic vesicle ISH
Prim-15 otic vesicle antero-medial region ISH
pou3f3b AB control Prim-15 otic vesicle postero-medial region ISH
pgk1x40/x40 control Prim-15 otic vesicle postero-medial region ISH
spry4 AB control 14-19 somites otic vesicle ISH
20-25 somites otic vesicle ISH
Prim-5 otic vesicle ISH
pgk1x40/x40 control 14-19 somites otic vesicle ISH
20-25 somites otic vesicle ISH
Prim-5 otic vesicle ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
pgk1x40/x40 control 14-19 somites otic vesicle etv5b expression decreased amount, abnormal
14-19 somites otic vesicle etv5b expression decreased distribution, abnormal
14-19 somites otic vesicle pax5 expression decreased amount, abnormal
14-19 somites otic vesicle spry4 expression decreased amount, abnormal
20-25 somites otic vesicle etv5b expression decreased amount, abnormal
20-25 somites otic vesicle etv5b expression decreased distribution, abnormal
20-25 somites otic vesicle pax5 expression decreased amount, abnormal
20-25 somites otic vesicle spry4 expression decreased amount, abnormal
Prim-5 anterior macula atoh1a expression decreased amount, abnormal
Prim-5 otic vesicle etv5b expression decreased amount, abnormal
Prim-5 otic vesicle etv5b expression decreased distribution, abnormal
Prim-5 otic vesicle pax5 expression decreased amount, abnormal
Prim-5 otic vesicle spry4 expression decreased amount, abnormal
Prim-5 posterior macula atoh1a expression decreased amount, abnormal
Prim-15 anterior macula atoh1a expression decreased amount, abnormal
Prim-15 posterior macula atoh1a expression decreased amount, abnormal
pgk1x40/x40; s356tTg control Prim-25 otic vesicle hair cell GFP expression decreased amount, abnormal
Prim-25 saccule hair cell decreased amount, abnormal
Prim-25 utricle hair cell decreased amount, abnormal
Long-pec otic vesicle hair cell GFP expression decreased amount, abnormal
Long-pec saccule hair cell decreased amount, abnormal
Long-pec utricle hair cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife