ZFIN ID: ZDB-FIG-200530-58
Kantarci et al., 2020 - The Warburg Effect and lactate signaling augment Fgf-MAPK to promote sensory-neural development in the otic vesicle. eLIFE   9 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-15
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: 14-19 somites to Day 5

Figure 4

Initial characterization of pgk1- / - mutants and genetic mosaics.

(A–F) Cross sections through the anterior/vestibular region of the otic vesicle (outlined) showing expression of the indicated genes in wild-type embryos and pgk1- / - mutants at the indicated times. (G–J) Lateral views showing isl2b-Gfp expression in live embryos at 30 hpf (G, H) and fgf3 at 24 hpf (I, J). The otic vesicle is outlined. (K–P) Cross sections through the otic vesicle (outlined) showing positions of lineage labeled wild-type cells (red dye) transplanted into a wild-type host (K, M, O) or a pgk1- / - mutant host (L, N, P). Sections are co-stained with DAPI (M, N) and etv5b probe (O, P). Note the absence of etv5bexpression in wild-type cells transplanted into the mutant host (P). (Q) Number of mature Isl1+ SAG neurons at 30 hpf in embryos with the indicated genotypes, except for sagd1 x pgk1 intercross expected to contain roughly 25% each of +/+, sagd1/+, pgk1/+ and sagd1/pgk1 embryos. (R) Percent of wild-type donor cells located in the ventral half of the otic vesicle expressing etv5b in wild-type or pgk1- / - hosts. A total of 151 wild-type donor cells were counted in eight otic vesicles of wild-type host embryos, and 247 wild-type donor cells were counted in eight otic vesicles of pgk1- / - host embryos. (S) Number of Isl1+ SAG neurons (total, anterior and posterior) in wild-type and pgk1- / - mutant embryos at 36 hpf (n = 12), 48 hpf (n = 12) and 120 hpf (n = 4). (T) Number of phalloidin-stained hair cells (anterior/utricular and posterior/saccular) in wild-type and pgk1- / - mutant embryos at 36 hpf (n = 16), 48 hpf (n = 16) and 120 hpf (n = 10). Sample sizes are indicated (S, T).


Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
etv5b AB control 14-19 somites otic vesicle ISH
pgk1x55/x55 control 14-19 somites otic vesicle ISH
fgf3 AB control Prim-5 otic vesicle ISH
pgk1x55/x55 control Prim-5 otic vesicle ISH
GFP pgk1x55/x55; zc7Tg control Prim-15 statoacoustic (VIII) ganglion IFL
zc7Tg control Prim-15 statoacoustic (VIII) ganglion IFL
neurod1 AB control Prim-5 otic vesicle ISH
pgk1x55/x55 control Prim-5 otic vesicle ISH
neurog1 AB control Prim-5 otic vesicle ISH
pgk1x55/x55 control Prim-5 otic vesicle ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
pgk1x40/x40; x65Tg; zc7Tg heat shock Prim-15 statoacoustic (VIII) ganglion has normal numbers of parts of type neuron, ameliorated
pgk1x40/x40; zc7Tg heat shock Prim-15 statoacoustic (VIII) ganglion has fewer parts of type neuron, abnormal
pgk1x55/x55 control 14-19 somites otic vesicle etv5b expression decreased amount, abnormal
Prim-5 otic vesicle neurod1 expression decreased amount, abnormal
Prim-5 otic vesicle neurog1 expression decreased amount, abnormal
pgk1x55/x55; zc7Tg control Prim-15 statoacoustic (VIII) ganglion has fewer parts of type neuron, abnormal
Prim-25 hair cell anterior macula decreased amount, abnormal
Prim-25 hair cell posterior macula decreased amount, abnormal
Prim-25 statoacoustic (VIII) ganglion anterior region has fewer parts of type neuron, abnormal
Prim-25 statoacoustic (VIII) ganglion posterior region has fewer parts of type neuron, abnormal
Long-pec hair cell anterior macula decreased amount, abnormal
Long-pec hair cell posterior macula decreased amount, abnormal
Long-pec statoacoustic (VIII) ganglion anterior region has fewer parts of type neuron, abnormal
Long-pec statoacoustic (VIII) ganglion posterior region has fewer parts of type neuron, abnormal
Day 5 hair cell anterior macula decreased amount, abnormal
Day 5 hair cell posterior macula decreased amount, abnormal
Day 5 statoacoustic (VIII) ganglion anterior region has fewer parts of type neuron, abnormal
Day 5 statoacoustic (VIII) ganglion posterior region has fewer parts of type neuron, abnormal
zc7Tg control Prim-15 statoacoustic (VIII) ganglion GFP expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife