ZFIN ID: ZDB-FIG-200306-123
Nimura et al., 2019 - Role of Reelin in cell positioning in the cerebellum and the cerebellum-like structure in zebrafish. Developmental Biology   455(2):393-408 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Days 30-44 to Adult
PHENOTYPE:
Fish:
Observed In:
Stage Range: Days 30-44 to Adult

Fig. 8

Localization of Reln protein in the tectum and the cerebellum. Medial and lateral sagittal sections of the brain from 30-dpf (A–P) and 90-dpf (Q–T) WT and relnΔ7/Δ7 mutant zebrafish were stained with anti-Reln (magenta), anti-Vglut1 (green), and Hoechst (cyan). Three fish for 30-dpf and one fish for 90-dpf WT or relnΔ7/Δ7 mutant fish were analyzed. Typical images are shown. (E-H, M-P) High magnification images of the boxes in C, D, K, and L. In WT, Reln protein was detected strongly in the SM and relatively weakly in the SO in the TeO. Reln was also detected strongly in the ML in the Cb. Weak Reln signals were also detected in the GCs in the TL and the GCL. These signals were absent in relnΔ7/Δ7mutants. CC, crista cerebellaris. The other abbreviations are described in the legend for Fig. 1. Scale bars: 100 μm in A (applies to A-D, I-L); 50 μm in E (applies to E-H, M-P); 200 μm in Q (applies to Q-T).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
reln Ab1-reln AB standard conditions Days 30-44 cerebellar crest IHC
Ab1-reln Days 30-44 cerebellar granule cell IHC
Ab1-reln Days 30-44 molecular layer corpus cerebelli IHC
Ab1-reln Days 30-44 stratum marginale IHC
Ab1-reln Days 30-44 stratum opticum IHC
Ab1-reln Days 30-44 torus longitudinalis granule cell IHC
Ab1-reln Adult cerebellar crest IHC
Ab1-reln Adult cerebellar granule cell IHC
Ab1-reln Adult molecular layer corpus cerebelli IHC
Ab1-reln Adult stratum marginale IHC
Ab1-reln Adult stratum opticum IHC
Ab1-reln Adult torus longitudinalis granule cell IHC
Ab1-reln relnnub23/nub23(AB) standard conditions Days 30-44 Not Detected cerebellar crest IHC
Ab1-reln Days 30-44 Not Detected cerebellar granule cell IHC
Ab1-reln Days 30-44 Not Detected molecular layer corpus cerebelli IHC
Ab1-reln Days 30-44 Not Detected stratum marginale IHC
Ab1-reln Days 30-44 Not Detected stratum opticum IHC
Ab1-reln Days 30-44 Not Detected torus longitudinalis granule cell IHC
Ab1-reln Adult Not Detected cerebellar crest IHC
Ab1-reln Adult Not Detected cerebellar granule cell IHC
Ab1-reln Adult Not Detected molecular layer corpus cerebelli IHC
Ab1-reln Adult Not Detected stratum marginale IHC
Ab1-reln Adult Not Detected stratum opticum IHC
Ab1-reln Adult Not Detected torus longitudinalis granule cell IHC
slc17a7a Ab1-slc17a7 AB standard conditions Days 30-44 molecular layer corpus cerebelli IHC
Ab1-slc17a7 Days 30-44 stratum marginale IHC
Ab1-slc17a7 Adult molecular layer corpus cerebelli IHC
Ab1-slc17a7 Adult stratum marginale IHC
Ab1-slc17a7 Adult torus longitudinalis IHC
Ab1-slc17a7 relnnub23/nub23(AB) standard conditions Days 30-44 molecular layer corpus cerebelli IHC
Ab1-slc17a7 Days 30-44 stratum marginale IHC
Ab1-slc17a7 Adult molecular layer corpus cerebelli IHC
Ab1-slc17a7 Adult stratum marginale IHC
Ab1-slc17a7 Adult torus longitudinalis IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
relnnub23/nub23(AB) standard conditions Days 30-44 cerebellar crest reln expression absent, abnormal
Days 30-44 cerebellar granule cell reln expression absent, abnormal
Days 30-44 molecular layer corpus cerebelli reln expression absent, abnormal
Days 30-44 stratum marginale reln expression absent, abnormal
Days 30-44 stratum opticum reln expression absent, abnormal
Days 30-44 torus longitudinalis granule cell reln expression absent, abnormal
Adult cerebellar crest reln expression absent, abnormal
Adult cerebellar granule cell reln expression absent, abnormal
Adult molecular layer corpus cerebelli reln expression absent, abnormal
Adult stratum marginale reln expression absent, abnormal
Adult stratum opticum reln expression absent, abnormal
Adult torus longitudinalis granule cell reln expression absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 455(2), Nimura, T., Itoh, T., Hagio, H., Hayashi, T., Di Donato, V., Takeuchi, M., Itoh, T., Inoguchi, F., Sato, Y., Yamamoto, N., Katsuyama, Y., Del Bene, F., Shimizu, T., Hibi, M., Role of Reelin in cell positioning in the cerebellum and the cerebellum-like structure in zebrafish, 393-408, Copyright (2019) with permission from Elsevier. Full text @ Dev. Biol.