ZFIN ID: ZDB-FIG-200306-121
Nimura et al., 2019 - Role of Reelin in cell positioning in the cerebellum and the cerebellum-like structure in zebrafish. Developmental Biology   455(2):393-408 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 6

Aberrant positioning of type I neurons in adult zebrafish relnvldlr, and dab1a mutants. (A–X) Cross sections of the brain from adult (90–150 dpf) WT (A-F, n= 9) and relnΔ7/Δ7 (G-L, n= 5), vldlr+13/+13 (M-R, n= 5), and dab1aΔ14/Δ14 (S-X, n= 4) mutant zebrafish were stained with anti- Pvalb7 (magenta) and anti-Vglut1 (green). Typical tectum images are shown. (D-F, J-L, P-R, V-X) High magnification images of the boxes in C, I, O, and U. Typical images are shown. Pvalb7+ type I neurons were located in a restricted area of the stratum fibrosum et griseum superficiale (SFGS) layer in the tectum in WT (A, D), but were ectopically localized in relnvldlr, and dab1a mutants (indicated by asterisks in G, J, M, P, S, and V). Dendrites of some type I neurons were misoriented (indicated by arrowheads in J, P, and V). The Vglut1+ axons of granule cells (GCs) in the tectum project to dendrites of type I neurons in the stratum marginale (SM) in WT (B, E). These axons projected to ectopic type I neurons in relnvldlr, and dab1a mutants (indicated by arrows in H, K, N, Q, T, and W). The stratum opticum (SO) is located between the SM and the SFGS. (Y) Position of type I neurons in WT (n= 9), relnΔ7/Δ7 (n= 4), vldlr+13/+13 (n= 4), and dab1aΔ14/Δ14 (n= 4). The tectum region was divided into ten domains from the pia to ventricle. The positions of the type I neurons were determined by measuring the distance between the pia and the soma of type I neurons. Pvalb7+ cells located in the vicinity of the ventricular zone that had a morphology different from type I neurons were excluded. Average and standard deviation are indicated in the graph. *p < 0.05; **p < 0.01; ***p< 0.001; ns not significant (Dunn’s multiple comparison test). Scale bars: 20 μm in A (applies to A-C, G-I, M-O, and S–U); 40 μm in D (applies to D-F, J-L, P-R, and V-X).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
pvalb7 Ab1-pvalb7 AB standard conditions Adult stratum fibrosum et griseum superficiale neuron IHC
Ab1-pvalb7 Adult stratum marginale IHC
Ab1-pvalb7 dab1aic3031/ic3031(AB) standard conditions Adult stratum fibrosum et griseum superficiale neuron IHC
Ab1-pvalb7 Adult stratum marginale IHC
Ab1-pvalb7 relnnub23/nub23(AB) standard conditions Adult stratum fibrosum et griseum superficiale neuron IHC
Ab1-pvalb7 Adult stratum marginale IHC
Ab1-pvalb7 vldlric3030/ic3030(AB) standard conditions Adult stratum fibrosum et griseum superficiale neuron IHC
Ab1-pvalb7 Adult stratum marginale IHC
slc17a7a Ab1-slc17a7 AB standard conditions Adult stratum marginale IHC
Ab1-slc17a7 dab1aic3031/ic3031(AB) standard conditions Adult stratum marginale IHC
Ab1-slc17a7 relnnub23/nub23(AB) standard conditions Adult stratum marginale IHC
Ab1-slc17a7 vldlric3030/ic3030(AB) standard conditions Adult stratum marginale IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
dab1aic3031/ic3031(AB) standard conditions Adult optic tectum neuron mislocalised radially, abnormal
relnnub23/nub23(AB) standard conditions Adult optic tectum neuron mislocalised radially, abnormal
vldlric3030/ic3030(AB) standard conditions Adult optic tectum neuron mislocalised radially, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 455(2), Nimura, T., Itoh, T., Hagio, H., Hayashi, T., Di Donato, V., Takeuchi, M., Itoh, T., Inoguchi, F., Sato, Y., Yamamoto, N., Katsuyama, Y., Del Bene, F., Shimizu, T., Hibi, M., Role of Reelin in cell positioning in the cerebellum and the cerebellum-like structure in zebrafish, 393-408, Copyright (2019) with permission from Elsevier. Full text @ Dev. Biol.