ZFIN ID: ZDB-FIG-200306-118
Nimura et al., 2019 - Role of Reelin in cell positioning in the cerebellum and the cerebellum-like structure in zebrafish. Developmental Biology   455(2):393-408 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 3

Projection of climbing fibers (CFs) to ectopic PCs in reln and dab1amutants. (A–L) Calretinin-immunoreactive (CR-ir+) axonal projections to PCs. Sagittal sections of adult (92-dpf) WT (A-D, n= 4) and relnΔ7/Δ7mutant (E-L, n= 4) brains were stained with anti-Pvalb7 (magenta) and anti-calretinin antibodies (green). (B-D, F–H, J-L) High magnification images of the boxes in A, E, and I. Typical images are shown. CR-ir+ axons projected to ectopic PCs located in the GCL in reln mutant cerebella, but not in WT cerebella. (M–T) CF projections to ectopic PCs. Sagittal section of adult (96 dpf) hspGFFDMC28C (28C); UAS:GFP fish brains, which express GFP in the CFs (axons of the neurons in the inferior olivary nuclei), harboring WT (control, n= 4) or homozygous reln mutant (relnΔ7/Δ7n= 4) alleles were stained with anti-Pvalb7 (magenta), and anti-GFP (green) antibodies. GFP+ CFs projected to ectopic PCs located in the GCL in reln mutant cerebella, but not in WT cerebella. Ectopic somata and dendrites of PCs are indicated by asterisks and arrowheads, respectively. CR-ir+ and 28C; UAS:GFP+ axons projecting to the ectopic Purkinje cells are indicated by arrows. The abbreviations are described in the legend for Fig. 1. Scale bars: 200 μm in A (applies to A, E, and I); 20 μm in B (applies to B-D, F–H, J-L); 200 μm in M (applies to M and Q); 20 μm in N (applies to N-P and R-T).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP nkhspGFFDMC28CEt ; nkuasgfp1aTg(AB) standard conditions Adult climbing fiber IHC
nkuasgfp1aTg ; relnnub23/nub23 ; nkhspGFFDMC28CEt(AB) standard conditions Adult climbing fiber IHC
pvalb7 Ab1-pvalb7 AB standard conditions Adult Purkinje cell cell body IHC
Ab1-pvalb7 Adult Purkinje cell neuron projection IHC
Ab1-pvalb7 dab1aic3031/ic3031(AB) standard conditions Adult Purkinje cell cell body IHC
Ab1-pvalb7 Adult Purkinje cell neuron projection IHC
Ab1-pvalb7 nkhspGFFDMC28CEt ; nkuasgfp1aTg(AB) standard conditions Adult Purkinje cell cell body IHC
Ab1-pvalb7 Adult Purkinje cell neuron projection IHC
Ab1-pvalb7 nkuasgfp1aTg ; relnnub23/nub23 ; nkhspGFFDMC28CEt(AB) standard conditions Adult Purkinje cell cell body IHC
Ab1-pvalb7 Adult Purkinje cell neuron projection IHC
Ab1-pvalb7 relnnub23/nub23(AB) standard conditions Adult Purkinje cell cell body IHC
Ab1-pvalb7 Adult Purkinje cell neuron projection IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab5-calb2 IHC AB standard conditions Adult climbing fiber
IHC dab1aic3031/ic3031(AB) standard conditions Adult climbing fiber
IHC relnnub23/nub23(AB) standard conditions Adult climbing fiber
Phenotype Details
Fish Conditions Stage Phenotype
dab1aic3031/ic3031(AB) standard conditions Adult granular layer corpus cerebelli has extra parts of type Purkinje cell, abnormal
Adult Purkinje cell inserted into granular layer corpus cerebelli, abnormal
nkuasgfp1aTg ; relnnub23/nub23 ; nkhspGFFDMC28CEt(AB) standard conditions Adult granular layer corpus cerebelli has extra parts of type Purkinje cell, abnormal
Adult Purkinje cell inserted into granular layer corpus cerebelli, abnormal
relnnub23/nub23(AB) standard conditions Adult granular layer corpus cerebelli has extra parts of type Purkinje cell, abnormal
Adult Purkinje cell inserted into granular layer corpus cerebelli, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 455(2), Nimura, T., Itoh, T., Hagio, H., Hayashi, T., Di Donato, V., Takeuchi, M., Itoh, T., Inoguchi, F., Sato, Y., Yamamoto, N., Katsuyama, Y., Del Bene, F., Shimizu, T., Hibi, M., Role of Reelin in cell positioning in the cerebellum and the cerebellum-like structure in zebrafish, 393-408, Copyright (2019) with permission from Elsevier. Full text @ Dev. Biol.