ZFIN ID: ZDB-FIG-200306-117
Nimura et al., 2019 - Role of Reelin in cell positioning in the cerebellum and the cerebellum-like structure in zebrafish. Developmental Biology   455(2):393-408 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 2

Aberrant positioning of Purkinje cells (PCs) in adult zebrafish relnvldlr, and dab1a mutants. (A–J) Sagittal sections of the brain from adult (90–150 dpf) wild-type (WT, A-A″, n= 6), and reln (C–C″, E-E″, n= 5 for relnΔ7/Δ7 and n= 5 for relnΔ28/Δ28), vldlr+13/+13 (G-G″, n= 5), and dab1aΔ14/Δ14(I–I″, n= 8) mutant zebrafish were stained with anti-parvalbumin7 (Pvalb7, magenta) and anti-Vglut1 (green) antibodies. Typical cerebellum images are shown. (B–B″, D-D″, F–F″, H–H″, J-J″) High magnification images of the boxes in A", C", E", G", and I". Ectopic Purkinje cells (PCs) are indicated by arrowheads. Axonal projections of granule cells (GCs) to ectopic PCs are marked by dotted circles. (K) Ectopic PCs. Sagittal sections 14-μm thick were prepared from adult zebrafish WT (n= 7), and relnΔ7/Δ7 (n= 8), vldlr+13/+13 (n= 5), and dab1aΔ14/Δ14 (n= 12) mutant brains. Pvalb7+ PCs in the granule cell layer (GCL) or the Purkinje cell layer (PCL) were counted in every fourth section (14 total sections near the midline of each fish). Average numbers and standard deviations of PCs in the GCL, PCL, or all layers (Total) are shown in the graph. (L) Proportion of PCs in the GCL in WT, and relnΔ7/Δ7vldlr+13/+13, and dab1aΔ14/Δ14 mutant cerebellum. A greater proportion of the total PCs were located in the GCL in the relnvldlr, and dab1a mutants than in WT. *p < 0.05; **p < 0.01; ***p< 0.001; ns not significant (Dunnett’s test for K and Dunn’s multiple comparison test for L). Scale bars: 100 μm in A (applies to A-A″, C–C″, E-E″, G-G″, I–I″); 50 μm in B, D, F, H, and J (applies to B–B″, D-D″, F–F″, H–H″ and J-J″, respectively).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
pvalb7 Ab1-pvalb7 AB standard conditions Adult Purkinje cell cell body IHC
Ab1-pvalb7 Adult Purkinje cell neuron projection IHC
Ab1-pvalb7 dab1aic3031/ic3031(AB) standard conditions Adult Purkinje cell cell body IHC
Ab1-pvalb7 Adult Purkinje cell neuron projection IHC
Ab1-pvalb7 relnic3032/ic3032(AB) standard conditions Adult Purkinje cell cell body IHC
Ab1-pvalb7 Adult Purkinje cell neuron projection IHC
Ab1-pvalb7 relnnub23/nub23(AB) standard conditions Adult Purkinje cell cell body IHC
Ab1-pvalb7 Adult Purkinje cell neuron projection IHC
Ab1-pvalb7 vldlric3030/ic3030(AB) standard conditions Adult Purkinje cell cell body IHC
Ab1-pvalb7 Adult Purkinje cell neuron projection IHC
slc17a7a Ab1-slc17a7 AB standard conditions Adult cerebellar granule cell axon IHC
Ab1-slc17a7 dab1aic3031/ic3031(AB) standard conditions Adult cerebellar granule cell axon IHC
Ab1-slc17a7 relnic3032/ic3032(AB) standard conditions Adult cerebellar granule cell axon IHC
Ab1-slc17a7 relnnub23/nub23(AB) standard conditions Adult cerebellar granule cell axon IHC
Ab1-slc17a7 vldlric3030/ic3030(AB) standard conditions Adult cerebellar granule cell axon IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
dab1aic3031/ic3031(AB) standard conditions Adult cerebellar granule cell differentiation normal occurrence, normal
Adult cerebellar Purkinje cell differentiation normal occurrence, normal
Adult cerebellar Purkinje cell layer morphogenesis decreased occurrence, abnormal
Adult granular layer corpus cerebelli has extra parts of type Purkinje cell, abnormal
Adult Purkinje cell inserted into granular layer corpus cerebelli, abnormal
Adult Purkinje cell layer corpus cerebelli has fewer parts of type Purkinje cell, abnormal
relnic3032/ic3032(AB) standard conditions Adult cerebellar granule cell differentiation normal occurrence, normal
Adult cerebellar Purkinje cell differentiation normal occurrence, normal
Adult cerebellar Purkinje cell layer morphogenesis decreased occurrence, abnormal
Adult granular layer corpus cerebelli has extra parts of type Purkinje cell, abnormal
Adult Purkinje cell inserted into granular layer corpus cerebelli, abnormal
Adult Purkinje cell layer corpus cerebelli has fewer parts of type Purkinje cell, abnormal
relnnub23/nub23(AB) standard conditions Adult cerebellar granule cell differentiation normal occurrence, normal
Adult cerebellar Purkinje cell differentiation normal occurrence, normal
Adult cerebellar Purkinje cell layer morphogenesis decreased occurrence, abnormal
Adult granular layer corpus cerebelli has extra parts of type Purkinje cell, abnormal
Adult Purkinje cell inserted into granular layer corpus cerebelli, abnormal
Adult Purkinje cell layer corpus cerebelli has fewer parts of type Purkinje cell, abnormal
vldlric3030/ic3030(AB) standard conditions Adult cerebellar granule cell differentiation normal occurrence, normal
Adult cerebellar Purkinje cell differentiation normal occurrence, normal
Adult cerebellar Purkinje cell layer morphogenesis decreased occurrence, abnormal
Adult granular layer corpus cerebelli has extra parts of type Purkinje cell, abnormal
Adult Purkinje cell inserted into granular layer corpus cerebelli, abnormal
Adult Purkinje cell layer corpus cerebelli has fewer parts of type Purkinje cell, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 455(2), Nimura, T., Itoh, T., Hagio, H., Hayashi, T., Di Donato, V., Takeuchi, M., Itoh, T., Inoguchi, F., Sato, Y., Yamamoto, N., Katsuyama, Y., Del Bene, F., Shimizu, T., Hibi, M., Role of Reelin in cell positioning in the cerebellum and the cerebellum-like structure in zebrafish, 393-408, Copyright (2019) with permission from Elsevier. Full text @ Dev. Biol.