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Fig. 6

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ZDB-FIG-180427-27

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Hui et al., 2017 - Zebrafish Regulatory T Cells Mediate Organ-Specific Regenerative Programs

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Fig. 6

zT_reg Cells Express Tissue-Specific Pro-regenerative Factors

(A–C) qRT-PCR analysis of growth factor expression in injured tissues (mean ± SEM, n = 6).

(D–F) qRT-PCR analysis of secreted factors in purified zT_reg cells (mean ± SD; ^∗p < 0.05, ^∗∗p < 0.01). Gene expression is shown relative to the levels in kidney-derived zT_reg cells.

(G) In situ hybridization using RNAscope and immunofluorescence against TagRFP in damaged foxp3a:RFP tissues. Arrows indicate zT_reg cells expressing mRNAs of ntf3 (left), nrg1(middle), or igf1(right). Single confocal sections are shown.

(H) Semi-qRT-PCR analysis of zT_reg cells purified from injured tissues and uninjured kidneys.

(I–K) Experimental interventions involving NTF3 (I), NRG1 (J), or IGF1 (K) treatments. i.p., intraperitoneal injection; i.v., intravitreal injection.

(L–N) Quantification of proliferating neural progenitor cells (L), cardiomyocytes (M), and Müller glia (N) (n = 5–8). Representative images are shown in Figures S4B, S4F, and S4G.

^∗p < 0.05, ^∗∗p < 0.01, Mann-Whitney U test (A–C and L–N) and Student's t test (D–F). Scale bars, 20 μm. See also Figure S4.

Expression Data

Genes:	areg egf gdnfa hbegfa igf1 igf2a igf2b il10 ngfb nr4a1 nrg1 ntf3 pdgfaa pdgfba
Fish:	vcc3Tg/+ vcc5Tg/+
Conditions:	cold damage: heart perforation: retina transection: spinal cord chemical ablation: T cell, chemical treatment by environment: metronidazole, cold damage: heart chemical ablation: T cell, chemical treatment by environment: metronidazole, perforation: retina chemical ablation: T cell, chemical treatment by environment: metronidazole, transection: spinal cord
Anatomical Terms:	regenerating tissue heart regenerating tissue retina regenerating tissue spinal cord regenerating tissue T cell
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	vcc5Tg/+
Conditions:	chemical ablation: T cell, chemical treatment by environment: metronidazole, cold damage: heart chemical ablation: T cell, chemical treatment by environment: metronidazole, perforation: retina chemical ablation: T cell, chemical treatment by environment: metronidazole, transection: spinal cord
Observed In:	regenerating tissue heart regenerating tissue retina regenerating tissue spinal cord
Stage:	Adult

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 43, Hui, S.P., Sheng, D.Z., Sugimoto, K., Gonzalez-Rajal, A., Nakagawa, S., Hesselson, D., Kikuchi, K., Zebrafish Regulatory T Cells Mediate Organ-Specific Regenerative Programs, 659-672.e5, Copyright (2017) with permission from Elsevier. Full text @ Dev. Cell