FIGURE

Fig. 7

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ZDB-FIG-180427-28

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Hui et al., 2017 - Zebrafish Regulatory T Cells Mediate Organ-Specific Regenerative Programs

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Fig. 7

Impaired Regeneration in Foxp3a-Deficient Zebrafish

(A) Foxp3a domain structure and predicted truncated product of the Foxp3a^vcc6 allele. Numbers indicate amino acid positions.

(B) Sections of Foxp3a-deficient (foxp3a^−/−) and WT (foxp3a^+/+) clutch-mate foxp3a:RFP fish. Asterisks indicate injury epicenter.

(C–E) Proliferation of newborn neurons (C), cardiomyocytes (D), and Müller glia (E). Insets show single-channel confocal slices (C and E) or epifluorescent images (D) of the demarcated regions. Arrows mark co-labeled cells. Dotted lines indicate the wound border.

(F–H) Quantification of (C), (D) and (E), respectively (mean ± SEM, n = 5–7).

(I–K) qRT-PCR analysis of regeneration factor genes (mean ± SEM, n = 6).

(L–N) Semi-qRT-PCR analysis of regeneration factor genes in purified foxp3a:RFP⁺ cells. +/+, foxp3a^+/+; −/−, foxp3a^−/−.

Confocal projections of z stacks are shown, except for the epifluorescent images in (D). ^∗p < 0.05, ^∗∗p < 0.01, Mann-Whitney U test. Scale bars, 50 μm. See also Figure S7.

Expression Data

Genes:	areg igf1 il10 nrg1 ntf3 TagRFP
Antibodies:	Ab-F59 Ab1-elavl Ab1-tuba
Fish:	foxp3a^vcc6/vcc6; vcc3Tg/+
Conditions:	cold damage: heart perforation: retina transection: spinal cord
Anatomical Terms:	regenerating tissue cardiac muscle cell regenerating tissue heart regenerating tissue neuroblast regenerating tissue neuron regenerating tissue retina regenerating tissue spinal cord regenerating tissue T cell
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	foxp3a^vcc6/vcc6; vcc3Tg/+
Conditions:	cold damage: heart perforation: retina transection: spinal cord
Observed In:	Muller cell cell population proliferation regenerating tissue cardiac muscle cell proliferation regenerating tissue cell population proliferation regenerating tissue heart regenerating tissue neuroblast proliferation regenerating tissue retina regenerating tissue spinal cord regenerating tissue T cell
Stage:	Adult

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 43, Hui, S.P., Sheng, D.Z., Sugimoto, K., Gonzalez-Rajal, A., Nakagawa, S., Hesselson, D., Kikuchi, K., Zebrafish Regulatory T Cells Mediate Organ-Specific Regenerative Programs, 659-672.e5, Copyright (2017) with permission from Elsevier. Full text @ Dev. Cell