Fig. S5

zT_reg cell-mediated immunomodulation during regeneration, Related to Figure 6. (A–C) qRT-PCR analysis of inflammatory gene expression in injured spinal cords (A), hearts (B), and retinas (C) from control and zT_reg celldepleted fish (mean ± SEM, n = 6). (D–F) Expression levels of the same genes analyzed in A–C were unchanged in the remainder of the fish after removing the injured spinal cord (D), heart (E), or retina (F) (mean ± SEM, n = 5). (G–I) The expression of M1 macrophage marker nos2b and M2 macrophage marker marco was measured in injured spinal cord (G), heart (H), and retina (I) samples obtained from Mtz-treated WT and foxp3a:NTR fish (mean ± SEM, n = 6). (J–L) Sections of spinal cords (J), hearts (K), and retinas (L) from lck:RFP (left) or lck:RFP; foxp3a:NTR fish (right) treated with Mtz (mean ± SEM, n = 6). Asterisks, injury epicenter. (M–O) Quantification of J (M), K (N), and L (O). zT_reg (+) and (−) indicate zT_reg cell ablated or non-ablated samples, respectively (mean ± SEM, n = 6). (P–R) qRT-PCR analysis of the expression of effector T cell marker genes in T cells (lck:RFP⁺ cells) purified from damaged spinal cords (P), hearts (Q), retinas (R) (mean ± SD; *P < 0.05). AcTub, Acetylated Tubulin; HT, heart; marco, macrophage receptor with collagenous domain; MHC, myosin heavy chain; nos2b, inducible nitric oxide synthase 2b; RT, retina; SC, spinal cord. *P < 0.05, **P < 0.01, ***P < 0.001, NS, not significant, Mann–Whitney U test (A–I, M–O), Student’s t-test (P–R). Scale bars, 100 μm.