FIGURE

Fig. 3

ID

ZDB-FIG-080323-3

Publication

Londin et al., 2007 - Churchill regulates cell movement and mesoderm specification by repressing Nodal signaling

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Fig. 3

chch inhibition results in decreased ectodermal gene expression. Real-time PCR analysis of mesodermal, ectodermal and endodermal markers during late gastrulation in chch-compromised embryos. The mesodermal markers flh, ntl, spt, and tbx6 (A), endodermal markers, mixer and sox17b (A) and ectodermal markers otx2, hoxb1b, sox3, krt8 and gata2 (B) were examined following microinjection of chch-EnR mRNA and chch-ATGMO. Mesodermal markers are increased, endodermal are unaffected while ectodermal marker levels are decreased. Expression of the neural genes otx2 at 90% epiboly (C-D) and 6-somites (E-F) and Six3 at 6-somites (G-H). C-D, G-H are dorsal views. E-F are lateral views.

Expression Data

Genes:	gata2a hoxb1b mixl1 noto otx2b six3a sox3 sox17 tbx16 tbx16l tbxta
Fish:	WT WT + MO1-churc1
Knockdown Reagent:	MO1-churc1
Anatomical Terms:	anterior neural plate ectoderm endoderm mesoderm neural plate
Stage Range:	90%-epiboly to 5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.