FIGURE

Fig. 4

ID

ZDB-FIG-201209-49

Publication

Zhao et al., 2020 - An optimized base editor with efficient C-to-T base editing in zebrafish

Other Figures

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Fig. 4

Generation of an AMS zebrafish model using the zAncBE4max system. a The designed target site in the twist2 gene on chromosome 9 in zebrafish. The nucleotide changes in C-to-T editing are underlined in red. The corresponding amino acid change is E78K. PAM region is indicated in blue. b Homozygous mutant (E78K) embryos exhibit a short truck and curved tail (red arrowheads) at 48 hpf. Scale bar, 100 μm. c Genotyping of homozygous mutant larva by PCR and sequencing. Red arrow indicates point mutation site C-to-T. d Phenotype of heterozygous mutants (E78K) exhibited a protruding jaw, unclosed mouth (red arrows), and emaciated body at the age of 6 months. For more details, see Additional file 3: Movie 1. e qRT-PCR analysis indicates the relative expression levels of twist2 and related genes in twist2 homozygous E78K mutant embryos

Expression Data

Genes:	alpl bmp2a bmp2b col1a2 il1b oscp1a runx2a runx2b sp7 tnfa twist2
Fish:	AB twist2^{zf3421/zf3421} (AB)
Anatomical Term:	whole organism
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	twist2^{zf3421/zf3421} (AB) twist2^zf3421/+ (AB)
Observed In:	mandibular arch skeleton mouth post-vent region trunk whole organism
Stage Range:	Long-pec to Adult

Phenotype Detail

Acknowledgments

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